Sagittula salina sp. nov., isolated from marine waste

A novel Gram-stain-negative, non-motile, halophilic bacterium designated strain M10.9X(T) was isolated from the inner sediment of an aluminium can collected from the Mediterranean Sea (València, Spain). Cells of strain M10.9X(T) were rod-shaped and occasionally formed aggregates. The strain was oxidase-negative and catalase-positive, and showed a slightly psychrophilic, neutrophilic and slightly halophilic metabolism. The phylogenetic analyses revealed that strain M10.9X(T) was closely related to Sagittula stellata E-37(T) and Sagittula marina F028-2(T). The genomic G+C content of strain M10.9X(T) was 65.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization values were 76.6 and 20.9 %, respectively, confirming its adscription to a new species within the genus Sagittula . The major cellular fatty acids were C(18 : 1) ω7c/C(18 : 1) ω6c and C(16 : 0). The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. According to the resuts of a polyphasic study, strain M10.9X(T) represents a novel species of the genus Sagittula for which the name Sagittula salina sp. nov. (type strain M10.9X(T)=DSM 112301(T)=CECT 30307(T)) is proposed.