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High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection
Intracytoplasmic sperm injection (ICSI) is an alternative technique to in vitro fertilization (IVF) for producing transferable blastocysts, especially in combination with cryopreserved oocytes, when the IVF system does not work sufficiently. The present study was conducted to directly compare the ef...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558808/ https://www.ncbi.nlm.nih.gov/pubmed/35965077 http://dx.doi.org/10.1262/jrd.2022-053 |
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author | HOCHI, Shinichi IDE, Misuzu UENO, Sayaka HIRABAYASHI, Masumi |
author_facet | HOCHI, Shinichi IDE, Misuzu UENO, Sayaka HIRABAYASHI, Masumi |
author_sort | HOCHI, Shinichi |
collection | PubMed |
description | Intracytoplasmic sperm injection (ICSI) is an alternative technique to in vitro fertilization (IVF) for producing transferable blastocysts, especially in combination with cryopreserved oocytes, when the IVF system does not work sufficiently. The present study was conducted to directly compare the efficacy of producing bovine blastocysts by ICSI and IVF from vitrified-warmed and fresh oocytes. Denuded oocytes with a detectable first polar body were vitrified-warmed using a nylon mesh device. In the non-vitrified control group, blastocyst yields 8 days after IVF and ICSI were 32.0 and 26.8%, respectively. Oocyte vitrification and subsequent IVF resulted in an impaired blastocyst yield (15.0%); however, such a loss of efficacy due to vitrification was not observed in the ICSI group (blastocyst yield, 25.2%). The alignment of cortical granules beneath the oolemma was comparable between the fresh control and vitrified-warmed oocytes. Here, we report the high survival of vitrified-warmed bovine oocytes, as assessed by ICSI. |
format | Online Article Text |
id | pubmed-9558808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-95588082022-11-03 High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection HOCHI, Shinichi IDE, Misuzu UENO, Sayaka HIRABAYASHI, Masumi J Reprod Dev Technology Report Intracytoplasmic sperm injection (ICSI) is an alternative technique to in vitro fertilization (IVF) for producing transferable blastocysts, especially in combination with cryopreserved oocytes, when the IVF system does not work sufficiently. The present study was conducted to directly compare the efficacy of producing bovine blastocysts by ICSI and IVF from vitrified-warmed and fresh oocytes. Denuded oocytes with a detectable first polar body were vitrified-warmed using a nylon mesh device. In the non-vitrified control group, blastocyst yields 8 days after IVF and ICSI were 32.0 and 26.8%, respectively. Oocyte vitrification and subsequent IVF resulted in an impaired blastocyst yield (15.0%); however, such a loss of efficacy due to vitrification was not observed in the ICSI group (blastocyst yield, 25.2%). The alignment of cortical granules beneath the oolemma was comparable between the fresh control and vitrified-warmed oocytes. Here, we report the high survival of vitrified-warmed bovine oocytes, as assessed by ICSI. The Society for Reproduction and Development 2022-08-15 2022-10 /pmc/articles/PMC9558808/ /pubmed/35965077 http://dx.doi.org/10.1262/jrd.2022-053 Text en ©2022 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Technology Report HOCHI, Shinichi IDE, Misuzu UENO, Sayaka HIRABAYASHI, Masumi High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title | High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title_full | High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title_fullStr | High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title_full_unstemmed | High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title_short | High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
title_sort | high survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558808/ https://www.ncbi.nlm.nih.gov/pubmed/35965077 http://dx.doi.org/10.1262/jrd.2022-053 |
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