Neospora spp. Seroprevalence and Risk Factors for Seropositivity in Apparently Healthy Horses and Pregnant Mares

SIMPLE SUMMARY: Neosporosis, caused by the parasite Neospora species, is recognized as one of the major causes of abortion in cattle worldwide causing large economic losses. Over the past few years, Neospora infection and parasite transmission from the mare to the fetus has been documented in horses and was associated with reproduction failure. In the present study, we investigated parasite prevalence and associated risk factors in the general equine population and in a group of pregnant mares during pregnancy and after parturition. Our findings revealed high exposure of horses to Neospora spp. parasites, with significantly higher prevalence in pregnant mares. ABSTRACT: Equine Neospora infection has been linked to neurological disorders and infertility in horses. This study looked into the risk factors for infection and the exposure to Neospora spp. in horses. The study was performed in two independent populations in Israel. The first consisted of apparently healthy horses, and the second consisted of mares examined during pregnancy and after parturition. Sera samples collected from horses and mares were tested for Neospora exposure by the indirect fluorescent antibody test (IFAT). The study revealed seroprevalence of 24% in apparently healthy horses and 66.4% and 48.6% in mares during gestation and after parturition, respectively. Among the investigated risk factors, older age (p = 0.026) and housing in both stalls and paddocks (p = 0.033) in apparently healthy horses, and Arabian breeds (p = 0.005) in pregnant mares, were found to be significantly associated with Neospora spp. seropositivity in univariable, but not multivariable, statistical analysis. This study revealed high exposure of equines to Neospora parasites, especially mares. Horse farm management, in combination with active surveillance, including serological testing and follow up, could help reduce the spread of the parasite among horses in endemic areas.