Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells

Proximity ligation assay (PLA) is a methodology that permits detection of protein-protein closeness, that is, proteins that are within 40 nanometers of each other, in cells or tissues at endogenous protein levels or after exogenous overexpression. It detects the protein(s) with high sensitivity and...

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Autores principales: Krieger, Christine C., Boutin, Alisa, Neumann, Susanne, Gershengorn, Marvin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Endocrinology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559199/
https://www.ncbi.nlm.nih.gov/pubmed/36246873
http://dx.doi.org/10.3389/fendo.2022.989626
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author Krieger, Christine C.
Boutin, Alisa
Neumann, Susanne
Gershengorn, Marvin C.
author_facet Krieger, Christine C.
Boutin, Alisa
Neumann, Susanne
Gershengorn, Marvin C.
author_sort Krieger, Christine C.
collection PubMed
description Proximity ligation assay (PLA) is a methodology that permits detection of protein-protein closeness, that is, proteins that are within 40 nanometers of each other, in cells or tissues at endogenous protein levels or after exogenous overexpression. It detects the protein(s) with high sensitivity and specificity because it employs a DNA hybridization step followed by DNA amplification. PLA has been used successfully with many types of proteins. In this methods paper, we will describe the workings of PLA and provide examples of its use to study TSH/IGF-1 receptor crosstalk in Graves’ orbital fibroblasts (GOFs) and TSH receptor homodimerization in primary cultures of human thyrocytes.
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spelling pubmed-95591992022-10-14 Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells Krieger, Christine C. Boutin, Alisa Neumann, Susanne Gershengorn, Marvin C. Front Endocrinol (Lausanne) Endocrinology Proximity ligation assay (PLA) is a methodology that permits detection of protein-protein closeness, that is, proteins that are within 40 nanometers of each other, in cells or tissues at endogenous protein levels or after exogenous overexpression. It detects the protein(s) with high sensitivity and specificity because it employs a DNA hybridization step followed by DNA amplification. PLA has been used successfully with many types of proteins. In this methods paper, we will describe the workings of PLA and provide examples of its use to study TSH/IGF-1 receptor crosstalk in Graves’ orbital fibroblasts (GOFs) and TSH receptor homodimerization in primary cultures of human thyrocytes. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9559199/ /pubmed/36246873 http://dx.doi.org/10.3389/fendo.2022.989626 Text en Copyright © 2022 Krieger, Boutin, Neumann and Gershengorn https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Krieger, Christine C.
Boutin, Alisa
Neumann, Susanne
Gershengorn, Marvin C.
Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title_full Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title_fullStr Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title_full_unstemmed Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title_short Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
title_sort proximity ligation assay to study tsh receptor homodimerization and crosstalk with igf-1 receptors in human thyroid cells
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559199/
https://www.ncbi.nlm.nih.gov/pubmed/36246873
http://dx.doi.org/10.3389/fendo.2022.989626
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