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Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement
SIMPLE SUMMARY: Lovastatin is a fungal secondary metabolite that can mitigate rumen methane production. This work aimed at evaluating the lovastatin production by solid-state fermentation from selected crop residues and A. terreus strains, considering the post-fermented residues as feed supplements...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559462/ https://www.ncbi.nlm.nih.gov/pubmed/36230438 http://dx.doi.org/10.3390/ani12192697 |
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author | Ábrego-García, Amaury Poggi-Varaldo, Héctor M. Ponce-Noyola, M. Teresa Calva-Calva, Graciano Galíndez-Mayer, Cutberto José Juvencio Medina-Mendoza, Gustavo G. Rinderknecht-Seijas, Noemí F. |
author_facet | Ábrego-García, Amaury Poggi-Varaldo, Héctor M. Ponce-Noyola, M. Teresa Calva-Calva, Graciano Galíndez-Mayer, Cutberto José Juvencio Medina-Mendoza, Gustavo G. Rinderknecht-Seijas, Noemí F. |
author_sort | Ábrego-García, Amaury |
collection | PubMed |
description | SIMPLE SUMMARY: Lovastatin is a fungal secondary metabolite that can mitigate rumen methane production. This work aimed at evaluating the lovastatin production by solid-state fermentation from selected crop residues and A. terreus strains, considering the post-fermented residues as feed supplements for ruminants. Fermented oat straw by A. terreus CDBB H-194 exhibited the highest lovastatin yield (23.8 mg/g DM fed). GC–MS analysis identified only a couple of compounds from the residues fermented by CDBB H-194 (1,3-dipalmitin trimethylsilyl ether in the fermented oat straw) and stearic acid hydrazide in the fermented wheat bran) that could negatively affect ruminal bacteria and fungi. ABSTRACT: This work aimed to evaluate the lovastatin (Lv) production by solid-state fermentation (SSF) from selected crop residues, considering the post-fermented residues as feed supplements for ruminants. The SSF was performed with two substrates (wheat bran and oat straw) and two A. terreus strains (CDBB H-194 and CDBB H-1976). The Lv yield, proximate analysis, and organic compounds by GC–MS in the post-fermented residues were assessed. The combination of the CDBB H-194 strain with oat straw at 16 d of incubation time showed the highest Lv yield (23.8 mg/g DM fed) and the corresponding degradation efficiency of hemicellulose + cellulose was low to moderate (24.1%). The other three treatments showed final Lv concentrations in decreasing order of 9.1, 6.8, and 5.67 mg/g DM fed for the oat straw + CDBB H-1976, wheat bran + CDBB H-194, and wheat bran + CDBB H-1976, respectively. An analysis of variance of the 2(2) factorial experiment of Lv showed a strong significant interaction between the strain and substrate factors. The kinetic of Lv production adequately fitted a zero-order model in the four treatments. GC–MS analysis identified only a couple of compounds from the residues fermented by A. terreus CDBB H-194 (1,3-dipalmitin trimethylsilyl ether in the fermented oat straw and stearic acid hydrazide in the fermented wheat bran) that could negatively affect ruminal bacteria and fungi. Solid-state fermentation of oat straw with CDBB H-194 deserves further investigation due to its high yield of Lv; low dietary proportions of this post-fermented oat straw could be used as an Lv-carrier supplement for rumen methane mitigation. |
format | Online Article Text |
id | pubmed-9559462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95594622022-10-14 Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement Ábrego-García, Amaury Poggi-Varaldo, Héctor M. Ponce-Noyola, M. Teresa Calva-Calva, Graciano Galíndez-Mayer, Cutberto José Juvencio Medina-Mendoza, Gustavo G. Rinderknecht-Seijas, Noemí F. Animals (Basel) Article SIMPLE SUMMARY: Lovastatin is a fungal secondary metabolite that can mitigate rumen methane production. This work aimed at evaluating the lovastatin production by solid-state fermentation from selected crop residues and A. terreus strains, considering the post-fermented residues as feed supplements for ruminants. Fermented oat straw by A. terreus CDBB H-194 exhibited the highest lovastatin yield (23.8 mg/g DM fed). GC–MS analysis identified only a couple of compounds from the residues fermented by CDBB H-194 (1,3-dipalmitin trimethylsilyl ether in the fermented oat straw) and stearic acid hydrazide in the fermented wheat bran) that could negatively affect ruminal bacteria and fungi. ABSTRACT: This work aimed to evaluate the lovastatin (Lv) production by solid-state fermentation (SSF) from selected crop residues, considering the post-fermented residues as feed supplements for ruminants. The SSF was performed with two substrates (wheat bran and oat straw) and two A. terreus strains (CDBB H-194 and CDBB H-1976). The Lv yield, proximate analysis, and organic compounds by GC–MS in the post-fermented residues were assessed. The combination of the CDBB H-194 strain with oat straw at 16 d of incubation time showed the highest Lv yield (23.8 mg/g DM fed) and the corresponding degradation efficiency of hemicellulose + cellulose was low to moderate (24.1%). The other three treatments showed final Lv concentrations in decreasing order of 9.1, 6.8, and 5.67 mg/g DM fed for the oat straw + CDBB H-1976, wheat bran + CDBB H-194, and wheat bran + CDBB H-1976, respectively. An analysis of variance of the 2(2) factorial experiment of Lv showed a strong significant interaction between the strain and substrate factors. The kinetic of Lv production adequately fitted a zero-order model in the four treatments. GC–MS analysis identified only a couple of compounds from the residues fermented by A. terreus CDBB H-194 (1,3-dipalmitin trimethylsilyl ether in the fermented oat straw and stearic acid hydrazide in the fermented wheat bran) that could negatively affect ruminal bacteria and fungi. Solid-state fermentation of oat straw with CDBB H-194 deserves further investigation due to its high yield of Lv; low dietary proportions of this post-fermented oat straw could be used as an Lv-carrier supplement for rumen methane mitigation. MDPI 2022-10-07 /pmc/articles/PMC9559462/ /pubmed/36230438 http://dx.doi.org/10.3390/ani12192697 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ábrego-García, Amaury Poggi-Varaldo, Héctor M. Ponce-Noyola, M. Teresa Calva-Calva, Graciano Galíndez-Mayer, Cutberto José Juvencio Medina-Mendoza, Gustavo G. Rinderknecht-Seijas, Noemí F. Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title | Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title_full | Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title_fullStr | Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title_full_unstemmed | Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title_short | Bioprocessing of Two Crop Residues for Animal Feeding into a High-Yield Lovastatin Feed Supplement |
title_sort | bioprocessing of two crop residues for animal feeding into a high-yield lovastatin feed supplement |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559462/ https://www.ncbi.nlm.nih.gov/pubmed/36230438 http://dx.doi.org/10.3390/ani12192697 |
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