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Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir

In this research antidiabetic, analgesic and antiulcer potential of traditional ethnomedicinal plant: Emex spinosa (L.) Campd. (Family Polygonaceae) was evaluated by extracting its phytoconstituents using methanol (MeOH) solvent through maceration protocol. The quantitative phytochemical analysis of...

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Autores principales: Ajaib, Muhammad, Ishtiaq, Saiqa, Ishtiaq, Muhammad, Maqbool, Mehwish, Bhatti, Khizar Hayat, Khan, Afsheen, Afreen, Afeera, Hussain, Tanveer, Sardar, Tauqeer, Gul, Alia, Azeem, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560475/
https://www.ncbi.nlm.nih.gov/pubmed/36227949
http://dx.doi.org/10.1371/journal.pone.0274706
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author Ajaib, Muhammad
Ishtiaq, Saiqa
Ishtiaq, Muhammad
Maqbool, Mehwish
Bhatti, Khizar Hayat
Khan, Afsheen
Afreen, Afeera
Hussain, Tanveer
Sardar, Tauqeer
Gul, Alia
Azeem, Muhammad
author_facet Ajaib, Muhammad
Ishtiaq, Saiqa
Ishtiaq, Muhammad
Maqbool, Mehwish
Bhatti, Khizar Hayat
Khan, Afsheen
Afreen, Afeera
Hussain, Tanveer
Sardar, Tauqeer
Gul, Alia
Azeem, Muhammad
author_sort Ajaib, Muhammad
collection PubMed
description In this research antidiabetic, analgesic and antiulcer potential of traditional ethnomedicinal plant: Emex spinosa (L.) Campd. (Family Polygonaceae) was evaluated by extracting its phytoconstituents using methanol (MeOH) solvent through maceration protocol. The quantitative phytochemical analysis of the extract revealed flavonoids were highest in leaf extract (15.63±0.93 mg/mL) and with (11.5±0.57 mg/mL) in stem. Alkaloids and tanins were also present in the samples in various conc. while saponins were absent. To confirm pharmaceutical potential of plant against ulcer, diabetes and analgesic infirmities, a model experimental animal wistar albino rats (Rattus norvegicus) were used. In antiulcer study, using hot plate method the maximum results were observed with 250 mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41, and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. For analysis of antiulcer potential of the plants parts doses of 250 and 500 mg/kg was applied to check the reclamation of ethanol-induced acute ulcer and of Aspirin-induced chronic ulcer of stomach. In order to confirm efficacy of the drug potential of plant following parameters like microscopic evaluation, gastric volume, total acidity, mucosa weight, ulcer index, pH and histopathology of stomach were analyzed. In antidiabetic analysis, in an acute study after a single dose of 500 mg/kg extract after 2hrs the blood glucose levels were 367±51.09958(NS), 416±59.79548(NS), 437.5±61.96437(NS) mg/dL for leaf, stem and fruit, respectively. After4hrs 351.75±88.27644(NS) mg/dl, 448.25±25.64948(NS) mg/dl, 445.25±27.07205(NS) mg/dl and after 6hrs 354.5±92.70428(NS), 442±24.60691(NS), a440±33.16625(NS) mg/dl, respectively. The analgesic activity was explored by applying hot plate, tail flick and formalin paw licking method. In hot plate method the maximum results were observed with 250mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41 and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. The respective plant extracts at 250mg/kg showed a gradual rise in latency time when compared to the control. It was concluded that all three components of E. spinosa perform proved to be significant as potential source of herbal medicines to cure different prevalently occurring diseases. Furthermore, it was confirmed through results analysis that plant t can be used to discover novel drug using dedicated high throughput techniques and ethnopharmacological approaches.
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spelling pubmed-95604752022-10-14 Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir Ajaib, Muhammad Ishtiaq, Saiqa Ishtiaq, Muhammad Maqbool, Mehwish Bhatti, Khizar Hayat Khan, Afsheen Afreen, Afeera Hussain, Tanveer Sardar, Tauqeer Gul, Alia Azeem, Muhammad PLoS One Research Article In this research antidiabetic, analgesic and antiulcer potential of traditional ethnomedicinal plant: Emex spinosa (L.) Campd. (Family Polygonaceae) was evaluated by extracting its phytoconstituents using methanol (MeOH) solvent through maceration protocol. The quantitative phytochemical analysis of the extract revealed flavonoids were highest in leaf extract (15.63±0.93 mg/mL) and with (11.5±0.57 mg/mL) in stem. Alkaloids and tanins were also present in the samples in various conc. while saponins were absent. To confirm pharmaceutical potential of plant against ulcer, diabetes and analgesic infirmities, a model experimental animal wistar albino rats (Rattus norvegicus) were used. In antiulcer study, using hot plate method the maximum results were observed with 250 mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41, and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. For analysis of antiulcer potential of the plants parts doses of 250 and 500 mg/kg was applied to check the reclamation of ethanol-induced acute ulcer and of Aspirin-induced chronic ulcer of stomach. In order to confirm efficacy of the drug potential of plant following parameters like microscopic evaluation, gastric volume, total acidity, mucosa weight, ulcer index, pH and histopathology of stomach were analyzed. In antidiabetic analysis, in an acute study after a single dose of 500 mg/kg extract after 2hrs the blood glucose levels were 367±51.09958(NS), 416±59.79548(NS), 437.5±61.96437(NS) mg/dL for leaf, stem and fruit, respectively. After4hrs 351.75±88.27644(NS) mg/dl, 448.25±25.64948(NS) mg/dl, 445.25±27.07205(NS) mg/dl and after 6hrs 354.5±92.70428(NS), 442±24.60691(NS), a440±33.16625(NS) mg/dl, respectively. The analgesic activity was explored by applying hot plate, tail flick and formalin paw licking method. In hot plate method the maximum results were observed with 250mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41 and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. The respective plant extracts at 250mg/kg showed a gradual rise in latency time when compared to the control. It was concluded that all three components of E. spinosa perform proved to be significant as potential source of herbal medicines to cure different prevalently occurring diseases. Furthermore, it was confirmed through results analysis that plant t can be used to discover novel drug using dedicated high throughput techniques and ethnopharmacological approaches. Public Library of Science 2022-10-13 /pmc/articles/PMC9560475/ /pubmed/36227949 http://dx.doi.org/10.1371/journal.pone.0274706 Text en © 2022 Ajaib et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ajaib, Muhammad
Ishtiaq, Saiqa
Ishtiaq, Muhammad
Maqbool, Mehwish
Bhatti, Khizar Hayat
Khan, Afsheen
Afreen, Afeera
Hussain, Tanveer
Sardar, Tauqeer
Gul, Alia
Azeem, Muhammad
Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title_full Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title_fullStr Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title_full_unstemmed Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title_short Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir
title_sort analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant emex spinosa (l.) campd. from azad jammu and kashmir
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560475/
https://www.ncbi.nlm.nih.gov/pubmed/36227949
http://dx.doi.org/10.1371/journal.pone.0274706
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