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Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis
This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560846/ https://www.ncbi.nlm.nih.gov/pubmed/36248056 http://dx.doi.org/10.1155/2022/2819855 |
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author | van der Burg, Debbie Wätzig, Hermann Sänger–van de Griend, Cari E. |
author_facet | van der Burg, Debbie Wätzig, Hermann Sänger–van de Griend, Cari E. |
author_sort | van der Burg, Debbie |
collection | PubMed |
description | This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10–110 µM for undiluted samples, with 10 μM being the lower range limit of quantification QL, showed accuracy recoveries of 94–104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively. |
format | Online Article Text |
id | pubmed-9560846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95608462022-10-14 Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis van der Burg, Debbie Wätzig, Hermann Sänger–van de Griend, Cari E. J Anal Methods Chem Research Article This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10–110 µM for undiluted samples, with 10 μM being the lower range limit of quantification QL, showed accuracy recoveries of 94–104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively. Hindawi 2022-10-06 /pmc/articles/PMC9560846/ /pubmed/36248056 http://dx.doi.org/10.1155/2022/2819855 Text en Copyright © 2022 Debbie van der Burg et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article van der Burg, Debbie Wätzig, Hermann Sänger–van de Griend, Cari E. Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title | Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title_full | Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title_fullStr | Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title_full_unstemmed | Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title_short | Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis |
title_sort | analysis of cationic vitamins in cell culture medium samples by capillary zone electrophoresis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560846/ https://www.ncbi.nlm.nih.gov/pubmed/36248056 http://dx.doi.org/10.1155/2022/2819855 |
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