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A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain

Severe Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. Among domestic animals, cats are more susceptible to SARS-CoV-2 than dogs. The detection of anti-SARS-CoV-2 antibodies in seemingly healthy cats and/or infected cats which are in close conta...

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Autores principales: Villanueva-Saz, Sergio, Martínez, Mariví, Giner, Jacobo, González, Ana, Tobajas, Ana Pilar, Pérez, María Dolores, Lira-Navarrete, Erandi, González-Ramírez, Andrés Manuel, Macías-León, Javier, Verde, Maite, Yzuel, Andrés, Hurtado-Guerrero, Ramón, Arias, Maykel, Santiago, Llipsy, Aguiló-Gisbert, Jordi, Ruíz, Héctor, Lacasta, Delia, Marteles, Diana, Fernández, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560875/
https://www.ncbi.nlm.nih.gov/pubmed/36229725
http://dx.doi.org/10.1007/s11259-022-10016-7
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author Villanueva-Saz, Sergio
Martínez, Mariví
Giner, Jacobo
González, Ana
Tobajas, Ana Pilar
Pérez, María Dolores
Lira-Navarrete, Erandi
González-Ramírez, Andrés Manuel
Macías-León, Javier
Verde, Maite
Yzuel, Andrés
Hurtado-Guerrero, Ramón
Arias, Maykel
Santiago, Llipsy
Aguiló-Gisbert, Jordi
Ruíz, Héctor
Lacasta, Delia
Marteles, Diana
Fernández, Antonio
author_facet Villanueva-Saz, Sergio
Martínez, Mariví
Giner, Jacobo
González, Ana
Tobajas, Ana Pilar
Pérez, María Dolores
Lira-Navarrete, Erandi
González-Ramírez, Andrés Manuel
Macías-León, Javier
Verde, Maite
Yzuel, Andrés
Hurtado-Guerrero, Ramón
Arias, Maykel
Santiago, Llipsy
Aguiló-Gisbert, Jordi
Ruíz, Héctor
Lacasta, Delia
Marteles, Diana
Fernández, Antonio
author_sort Villanueva-Saz, Sergio
collection PubMed
description Severe Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. Among domestic animals, cats are more susceptible to SARS-CoV-2 than dogs. The detection of anti-SARS-CoV-2 antibodies in seemingly healthy cats and/or infected cats which are in close contact with infected humans has been described. The presence of animals that tested positive by serology or molecular techniques could represent a potential transmission pathway of SARS-CoV-2 that can spill over into urban wildlife. This study analyses the seroprevalence variation of SARS-CoV-2 in stray cats from different waves of outbreaks in a geographical area where previous seroepidemiological information of SARS-CoV-2 was available and investigate if SARS-CoV-2-seropositive cats were exposed to other co-infections causing an immunosuppressive status and/or a chronic disease that could lead to a SARS-CoV-2 susceptibility. For this purpose, a total of 254 stray cats from Zaragoza (Spain) were included. This analysis was carried out by the enzyme-linked immunosorbent assay using the receptor binding domain of Spike antigen and confirmed by serum virus neutralization assay. The presence of co-infections including Toxoplasma gondii, Leishmania infantum, Dirofilaria immitis, feline calicivirus, feline herpesvirus type 1, feline leukemia virus and feline immunodeficiency virus, was evaluated using different serological methods. A seropositivity of 1.57% was observed for SARS-CoV-2 including the presence of neutralizing antibodies in three cats. None of the seropositive to SARS-CoV-2 cats were positive to feline coronavirus, however, four SARS-CoV-2-seropositive cats were also seropositive to other pathogens such as L. infantum, D. immitis and FIV (n = 1), L. infantum and D. immitis (n = 1) and L. infantum alone (n = 1).Considering other pathogens, a seroprevalence of 16.54% was detected for L. infantum, 30.31% for D. immitis, 13.78%, for T. gondii, 83.86% for feline calicivirus, 42.52% for feline herpesvirus type 1, 3.15% for FeLV and 7.87% for FIV. Our findings suggest that the epidemiological role of stray cats in SARS-CoV-2 transmission is scarce, and there is no increase in seropositivity during the different waves of COVID-19 outbreaks in this group of animals. Further epidemiological surveillances are necessary to determine the risk that other animals might possess even though stray cats do not seem to play a role in transmission.
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spelling pubmed-95608752022-10-14 A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain Villanueva-Saz, Sergio Martínez, Mariví Giner, Jacobo González, Ana Tobajas, Ana Pilar Pérez, María Dolores Lira-Navarrete, Erandi González-Ramírez, Andrés Manuel Macías-León, Javier Verde, Maite Yzuel, Andrés Hurtado-Guerrero, Ramón Arias, Maykel Santiago, Llipsy Aguiló-Gisbert, Jordi Ruíz, Héctor Lacasta, Delia Marteles, Diana Fernández, Antonio Vet Res Commun Research Severe Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. Among domestic animals, cats are more susceptible to SARS-CoV-2 than dogs. The detection of anti-SARS-CoV-2 antibodies in seemingly healthy cats and/or infected cats which are in close contact with infected humans has been described. The presence of animals that tested positive by serology or molecular techniques could represent a potential transmission pathway of SARS-CoV-2 that can spill over into urban wildlife. This study analyses the seroprevalence variation of SARS-CoV-2 in stray cats from different waves of outbreaks in a geographical area where previous seroepidemiological information of SARS-CoV-2 was available and investigate if SARS-CoV-2-seropositive cats were exposed to other co-infections causing an immunosuppressive status and/or a chronic disease that could lead to a SARS-CoV-2 susceptibility. For this purpose, a total of 254 stray cats from Zaragoza (Spain) were included. This analysis was carried out by the enzyme-linked immunosorbent assay using the receptor binding domain of Spike antigen and confirmed by serum virus neutralization assay. The presence of co-infections including Toxoplasma gondii, Leishmania infantum, Dirofilaria immitis, feline calicivirus, feline herpesvirus type 1, feline leukemia virus and feline immunodeficiency virus, was evaluated using different serological methods. A seropositivity of 1.57% was observed for SARS-CoV-2 including the presence of neutralizing antibodies in three cats. None of the seropositive to SARS-CoV-2 cats were positive to feline coronavirus, however, four SARS-CoV-2-seropositive cats were also seropositive to other pathogens such as L. infantum, D. immitis and FIV (n = 1), L. infantum and D. immitis (n = 1) and L. infantum alone (n = 1).Considering other pathogens, a seroprevalence of 16.54% was detected for L. infantum, 30.31% for D. immitis, 13.78%, for T. gondii, 83.86% for feline calicivirus, 42.52% for feline herpesvirus type 1, 3.15% for FeLV and 7.87% for FIV. Our findings suggest that the epidemiological role of stray cats in SARS-CoV-2 transmission is scarce, and there is no increase in seropositivity during the different waves of COVID-19 outbreaks in this group of animals. Further epidemiological surveillances are necessary to determine the risk that other animals might possess even though stray cats do not seem to play a role in transmission. Springer Netherlands 2022-10-14 2023 /pmc/articles/PMC9560875/ /pubmed/36229725 http://dx.doi.org/10.1007/s11259-022-10016-7 Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research
Villanueva-Saz, Sergio
Martínez, Mariví
Giner, Jacobo
González, Ana
Tobajas, Ana Pilar
Pérez, María Dolores
Lira-Navarrete, Erandi
González-Ramírez, Andrés Manuel
Macías-León, Javier
Verde, Maite
Yzuel, Andrés
Hurtado-Guerrero, Ramón
Arias, Maykel
Santiago, Llipsy
Aguiló-Gisbert, Jordi
Ruíz, Héctor
Lacasta, Delia
Marteles, Diana
Fernández, Antonio
A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title_full A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title_fullStr A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title_full_unstemmed A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title_short A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain
title_sort cross-sectional serosurvey of sars-cov-2 and co-infections in stray cats from the second wave to the sixth wave of covid-19 outbreaks in spain
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560875/
https://www.ncbi.nlm.nih.gov/pubmed/36229725
http://dx.doi.org/10.1007/s11259-022-10016-7
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