An “on-off-on” fluorescence probe for glyphosate detection based on Cu(2+) modulated g-C(3)N(4) nanosheets

The analysis of glyphosate is essential to agricultural production, environment protection and public health. Herein, we proposed a fast and convenient “on-off-on” fluorescence platform for sensitive detection of glyphosate via Cu(2+) modulated g-C(3)N(4) nanosheets. The fluorescence of the system was quenched by Cu(2+). With the presence of glyphosate, the fluorescence could be restored due to the formation of Cu(2+)- glyphosate complex. The proposed method was cost-effective with label-free and enzyme-free. Moreover, it exhibits high sensitivity with a low detection limit of 0.01 μg/ml. Furthermore, the proposed method has been successfully monitored glyphosate in real samples.