Protein delivery to living cells by thermal stimulation for biophysical investigation

Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of t...

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Autores principales: Torricella, Francesco, Barbieri, Letizia, Bazzurro, Virginia, Diaspro, Alberto, Banci, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561116/
https://www.ncbi.nlm.nih.gov/pubmed/36229511
http://dx.doi.org/10.1038/s41598-022-21103-9
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author Torricella, Francesco
Barbieri, Letizia
Bazzurro, Virginia
Diaspro, Alberto
Banci, Lucia
author_facet Torricella, Francesco
Barbieri, Letizia
Bazzurro, Virginia
Diaspro, Alberto
Banci, Lucia
author_sort Torricella, Francesco
collection PubMed
description Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of this approach for delivering proteins in the intracellular environment of mammalian cells reaching a concentration range suitable for successfully applying biophysical methods, such as double electron electron resonance (DEER) measurements for characterising protein conformations.
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spelling pubmed-95611162022-10-15 Protein delivery to living cells by thermal stimulation for biophysical investigation Torricella, Francesco Barbieri, Letizia Bazzurro, Virginia Diaspro, Alberto Banci, Lucia Sci Rep Article Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of this approach for delivering proteins in the intracellular environment of mammalian cells reaching a concentration range suitable for successfully applying biophysical methods, such as double electron electron resonance (DEER) measurements for characterising protein conformations. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9561116/ /pubmed/36229511 http://dx.doi.org/10.1038/s41598-022-21103-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Torricella, Francesco
Barbieri, Letizia
Bazzurro, Virginia
Diaspro, Alberto
Banci, Lucia
Protein delivery to living cells by thermal stimulation for biophysical investigation
title Protein delivery to living cells by thermal stimulation for biophysical investigation
title_full Protein delivery to living cells by thermal stimulation for biophysical investigation
title_fullStr Protein delivery to living cells by thermal stimulation for biophysical investigation
title_full_unstemmed Protein delivery to living cells by thermal stimulation for biophysical investigation
title_short Protein delivery to living cells by thermal stimulation for biophysical investigation
title_sort protein delivery to living cells by thermal stimulation for biophysical investigation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561116/
https://www.ncbi.nlm.nih.gov/pubmed/36229511
http://dx.doi.org/10.1038/s41598-022-21103-9
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