Cargando…
Protein delivery to living cells by thermal stimulation for biophysical investigation
Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of t...
Autores principales: | Torricella, Francesco, Barbieri, Letizia, Bazzurro, Virginia, Diaspro, Alberto, Banci, Lucia |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561116/ https://www.ncbi.nlm.nih.gov/pubmed/36229511 http://dx.doi.org/10.1038/s41598-022-21103-9 |
Ejemplares similares
-
Protein interaction patterns in different cellular environments are revealed by in-cell NMR
por: Barbieri, Letizia, et al.
Publicado: (2015) -
Atomic-resolution monitoring of protein maturation in live human cells by NMR
por: Banci, Lucia, et al.
Publicado: (2013) -
Protein in-cell NMR spectroscopy at 1.2 GHz
por: Luchinat, Enrico, et al.
Publicado: (2021) -
Precise 3D modulation of electro-optical parameters during neurotransmitter uncaging experiments with neurons in vitro
por: Cozzolino, Marco, et al.
Publicado: (2020) -
Involvement of GABA(A) receptors containing α(6) subtypes in antisecretory factor activity on rat cerebellar granule cells studied by two‐photon uncaging
por: Bazzurro, Virginia, et al.
Publicado: (2022)