Assessment of variability in the plasma 7k SomaScan proteomics assay

SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we pres...

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Autores principales: Candia, Julián, Daya, Gulzar N., Tanaka, Toshiko, Ferrucci, Luigi, Walker, Keenan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561184/
https://www.ncbi.nlm.nih.gov/pubmed/36229504
http://dx.doi.org/10.1038/s41598-022-22116-0
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author Candia, Julián
Daya, Gulzar N.
Tanaka, Toshiko
Ferrucci, Luigi
Walker, Keenan A.
author_facet Candia, Julián
Daya, Gulzar N.
Tanaka, Toshiko
Ferrucci, Luigi
Walker, Keenan A.
author_sort Candia, Julián
collection PubMed
description SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.
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spelling pubmed-95611842022-10-15 Assessment of variability in the plasma 7k SomaScan proteomics assay Candia, Julián Daya, Gulzar N. Tanaka, Toshiko Ferrucci, Luigi Walker, Keenan A. Sci Rep Article SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9561184/ /pubmed/36229504 http://dx.doi.org/10.1038/s41598-022-22116-0 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Candia, Julián
Daya, Gulzar N.
Tanaka, Toshiko
Ferrucci, Luigi
Walker, Keenan A.
Assessment of variability in the plasma 7k SomaScan proteomics assay
title Assessment of variability in the plasma 7k SomaScan proteomics assay
title_full Assessment of variability in the plasma 7k SomaScan proteomics assay
title_fullStr Assessment of variability in the plasma 7k SomaScan proteomics assay
title_full_unstemmed Assessment of variability in the plasma 7k SomaScan proteomics assay
title_short Assessment of variability in the plasma 7k SomaScan proteomics assay
title_sort assessment of variability in the plasma 7k somascan proteomics assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561184/
https://www.ncbi.nlm.nih.gov/pubmed/36229504
http://dx.doi.org/10.1038/s41598-022-22116-0
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