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Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex

Long noncoding RNAs (lncRNAs) play important roles in the spatial and temporal regulation of muscle development and regeneration. Nevertheless, the determination of their biological functions and mechanisms underlying muscle regeneration remains challenging. Here, we identified a lncRNA named lncMRE...

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Autores principales: Lv, Wei, Jiang, Wei, Luo, Hongmei, Tong, Qian, Niu, Xiaoyu, Liu, Xiao, Miao, Yang, Wang, Jingnan, Guo, Yiwen, Li, Jianan, Zhan, Xizhen, Hou, Yunqing, Peng, Yaxin, Wang, Jian, Zhao, Shuhong, Xu, Zaiyan, Zuo, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561262/
https://www.ncbi.nlm.nih.gov/pubmed/36200826
http://dx.doi.org/10.1093/nar/gkac854
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author Lv, Wei
Jiang, Wei
Luo, Hongmei
Tong, Qian
Niu, Xiaoyu
Liu, Xiao
Miao, Yang
Wang, Jingnan
Guo, Yiwen
Li, Jianan
Zhan, Xizhen
Hou, Yunqing
Peng, Yaxin
Wang, Jian
Zhao, Shuhong
Xu, Zaiyan
Zuo, Bo
author_facet Lv, Wei
Jiang, Wei
Luo, Hongmei
Tong, Qian
Niu, Xiaoyu
Liu, Xiao
Miao, Yang
Wang, Jingnan
Guo, Yiwen
Li, Jianan
Zhan, Xizhen
Hou, Yunqing
Peng, Yaxin
Wang, Jian
Zhao, Shuhong
Xu, Zaiyan
Zuo, Bo
author_sort Lv, Wei
collection PubMed
description Long noncoding RNAs (lncRNAs) play important roles in the spatial and temporal regulation of muscle development and regeneration. Nevertheless, the determination of their biological functions and mechanisms underlying muscle regeneration remains challenging. Here, we identified a lncRNA named lncMREF (lncRNA muscle regeneration enhancement factor) as a conserved positive regulator of muscle regeneration among mice, pigs and humans. Functional studies demonstrated that lncMREF, which is mainly expressed in differentiated muscle satellite cells, promotes myogenic differentiation and muscle regeneration. Mechanistically, lncMREF interacts with Smarca5 to promote chromatin accessibility when muscle satellite cells are activated and start to differentiate, thereby facilitating genomic binding of p300/CBP/H3K27ac to upregulate the expression of myogenic regulators, such as MyoD and cell differentiation. Our results unravel a novel temporal-specific epigenetic regulation during muscle regeneration and reveal that lncMREF/Smarca5-mediated epigenetic programming is responsible for muscle cell differentiation, which provides new insights into the regulatory mechanism of muscle regeneration.
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spelling pubmed-95612622022-10-18 Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex Lv, Wei Jiang, Wei Luo, Hongmei Tong, Qian Niu, Xiaoyu Liu, Xiao Miao, Yang Wang, Jingnan Guo, Yiwen Li, Jianan Zhan, Xizhen Hou, Yunqing Peng, Yaxin Wang, Jian Zhao, Shuhong Xu, Zaiyan Zuo, Bo Nucleic Acids Res RNA and RNA-protein complexes Long noncoding RNAs (lncRNAs) play important roles in the spatial and temporal regulation of muscle development and regeneration. Nevertheless, the determination of their biological functions and mechanisms underlying muscle regeneration remains challenging. Here, we identified a lncRNA named lncMREF (lncRNA muscle regeneration enhancement factor) as a conserved positive regulator of muscle regeneration among mice, pigs and humans. Functional studies demonstrated that lncMREF, which is mainly expressed in differentiated muscle satellite cells, promotes myogenic differentiation and muscle regeneration. Mechanistically, lncMREF interacts with Smarca5 to promote chromatin accessibility when muscle satellite cells are activated and start to differentiate, thereby facilitating genomic binding of p300/CBP/H3K27ac to upregulate the expression of myogenic regulators, such as MyoD and cell differentiation. Our results unravel a novel temporal-specific epigenetic regulation during muscle regeneration and reveal that lncMREF/Smarca5-mediated epigenetic programming is responsible for muscle cell differentiation, which provides new insights into the regulatory mechanism of muscle regeneration. Oxford University Press 2022-10-06 /pmc/articles/PMC9561262/ /pubmed/36200826 http://dx.doi.org/10.1093/nar/gkac854 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA and RNA-protein complexes
Lv, Wei
Jiang, Wei
Luo, Hongmei
Tong, Qian
Niu, Xiaoyu
Liu, Xiao
Miao, Yang
Wang, Jingnan
Guo, Yiwen
Li, Jianan
Zhan, Xizhen
Hou, Yunqing
Peng, Yaxin
Wang, Jian
Zhao, Shuhong
Xu, Zaiyan
Zuo, Bo
Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title_full Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title_fullStr Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title_full_unstemmed Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title_short Long noncoding RNA lncMREF promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex
title_sort long noncoding rna lncmref promotes myogenic differentiation and muscle regeneration by interacting with the smarca5/p300 complex
topic RNA and RNA-protein complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561262/
https://www.ncbi.nlm.nih.gov/pubmed/36200826
http://dx.doi.org/10.1093/nar/gkac854
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