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Negative supercoils regulate meiotic crossover patterns in budding yeast

Interference exists ubiquitously in many biological processes. Crossover interference patterns meiotic crossovers, which are required for faithful chromosome segregation and evolutionary adaption. However, what the interference signal is and how it is generated and regulated is unknown. We show that...

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Autores principales: Tan, Taicong, Tan, Yingjin, Wang, Ying, Yang, Xiao, Zhai, Binyuan, Zhang, Shuxian, Yang, Xuan, Nie, Hui, Gao, Jinmin, Zhou, Jun, Zhang, Liangran, Wang, Shunxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561271/
https://www.ncbi.nlm.nih.gov/pubmed/36107772
http://dx.doi.org/10.1093/nar/gkac786
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author Tan, Taicong
Tan, Yingjin
Wang, Ying
Yang, Xiao
Zhai, Binyuan
Zhang, Shuxian
Yang, Xuan
Nie, Hui
Gao, Jinmin
Zhou, Jun
Zhang, Liangran
Wang, Shunxin
author_facet Tan, Taicong
Tan, Yingjin
Wang, Ying
Yang, Xiao
Zhai, Binyuan
Zhang, Shuxian
Yang, Xuan
Nie, Hui
Gao, Jinmin
Zhou, Jun
Zhang, Liangran
Wang, Shunxin
author_sort Tan, Taicong
collection PubMed
description Interference exists ubiquitously in many biological processes. Crossover interference patterns meiotic crossovers, which are required for faithful chromosome segregation and evolutionary adaption. However, what the interference signal is and how it is generated and regulated is unknown. We show that yeast top2 alleles which cannot bind or cleave DNA accumulate a higher level of negative supercoils and show weaker interference. However, top2 alleles which cannot religate the cleaved DNA or release the religated DNA accumulate less negative supercoils and show stronger interference. Moreover, the level of negative supercoils is negatively correlated with crossover interference strength. Furthermore, negative supercoils preferentially enrich at crossover-associated Zip3 regions before the formation of meiotic DNA double-strand breaks, and regions with more negative supercoils tend to have more Zip3. Additionally, the strength of crossover interference and homeostasis change coordinately in mutants. These findings suggest that the accumulation and relief of negative supercoils pattern meiotic crossovers.
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spelling pubmed-95612712022-10-18 Negative supercoils regulate meiotic crossover patterns in budding yeast Tan, Taicong Tan, Yingjin Wang, Ying Yang, Xiao Zhai, Binyuan Zhang, Shuxian Yang, Xuan Nie, Hui Gao, Jinmin Zhou, Jun Zhang, Liangran Wang, Shunxin Nucleic Acids Res Genome Integrity, Repair and Replication Interference exists ubiquitously in many biological processes. Crossover interference patterns meiotic crossovers, which are required for faithful chromosome segregation and evolutionary adaption. However, what the interference signal is and how it is generated and regulated is unknown. We show that yeast top2 alleles which cannot bind or cleave DNA accumulate a higher level of negative supercoils and show weaker interference. However, top2 alleles which cannot religate the cleaved DNA or release the religated DNA accumulate less negative supercoils and show stronger interference. Moreover, the level of negative supercoils is negatively correlated with crossover interference strength. Furthermore, negative supercoils preferentially enrich at crossover-associated Zip3 regions before the formation of meiotic DNA double-strand breaks, and regions with more negative supercoils tend to have more Zip3. Additionally, the strength of crossover interference and homeostasis change coordinately in mutants. These findings suggest that the accumulation and relief of negative supercoils pattern meiotic crossovers. Oxford University Press 2022-09-15 /pmc/articles/PMC9561271/ /pubmed/36107772 http://dx.doi.org/10.1093/nar/gkac786 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Genome Integrity, Repair and Replication
Tan, Taicong
Tan, Yingjin
Wang, Ying
Yang, Xiao
Zhai, Binyuan
Zhang, Shuxian
Yang, Xuan
Nie, Hui
Gao, Jinmin
Zhou, Jun
Zhang, Liangran
Wang, Shunxin
Negative supercoils regulate meiotic crossover patterns in budding yeast
title Negative supercoils regulate meiotic crossover patterns in budding yeast
title_full Negative supercoils regulate meiotic crossover patterns in budding yeast
title_fullStr Negative supercoils regulate meiotic crossover patterns in budding yeast
title_full_unstemmed Negative supercoils regulate meiotic crossover patterns in budding yeast
title_short Negative supercoils regulate meiotic crossover patterns in budding yeast
title_sort negative supercoils regulate meiotic crossover patterns in budding yeast
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561271/
https://www.ncbi.nlm.nih.gov/pubmed/36107772
http://dx.doi.org/10.1093/nar/gkac786
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