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Efficient quantitative monitoring of translational initiation by RelE cleavage
The sequences of the 5′ untranslated regions (5′-UTRs) of mRNA alter gene expression across domains of life. Transcriptional modulators can be easily assayed through transcription termination, but translational regulators often require indirect, laborious methods. We have leveraged RelE’s ribosome-d...
Autores principales: | Focht, Caroline M, Strobel, Scott A |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561414/ https://www.ncbi.nlm.nih.gov/pubmed/35871288 http://dx.doi.org/10.1093/nar/gkac614 |
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