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Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior
Understanding the genomic underpinnings of antipredatory behaviors is a hot topic in eco-evolutionary research. Yellow-bellied toad of the genus Bombina are textbook examples of the deimatic display, a time-structured behavior aimed at startling predators. Here, we generated the first de novo brain...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561626/ https://www.ncbi.nlm.nih.gov/pubmed/36229462 http://dx.doi.org/10.1038/s41597-022-01724-5 |
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author | Chiocchio, Andrea Libro, Pietro Martino, Giuseppe Bisconti, Roberta Castrignanò, Tiziana Canestrelli, Daniele |
author_facet | Chiocchio, Andrea Libro, Pietro Martino, Giuseppe Bisconti, Roberta Castrignanò, Tiziana Canestrelli, Daniele |
author_sort | Chiocchio, Andrea |
collection | PubMed |
description | Understanding the genomic underpinnings of antipredatory behaviors is a hot topic in eco-evolutionary research. Yellow-bellied toad of the genus Bombina are textbook examples of the deimatic display, a time-structured behavior aimed at startling predators. Here, we generated the first de novo brain transcriptome of the Apennine yellow-bellied toad Bombina pachypus, a species showing inter-individual variation in the deimatic display. Through Rna-Seq experiments on a set of individuals showing distinct behavioral phenotypes, we generated 316,329,573 reads, which were assembled and annotated. The high-quality assembly was confirmed by assembly validators and by aligning the contigs against the de novo transcriptome with a mapping percentage higher than 91.0%. The homology annotation with DIAMOND (blastx) led to 77,391 contigs annotated on Nr, Swiss Prot and TrEMBL, whereas the domain and site protein prediction made with InterProScan led to 4747 GO-annotated and 1025 KEGG-annotated contigs. The B. pachypus transcriptome described here will be a valuable resource for further studies on the genomic underpinnings of behavioral variation in amphibians. |
format | Online Article Text |
id | pubmed-9561626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95616262022-10-15 Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior Chiocchio, Andrea Libro, Pietro Martino, Giuseppe Bisconti, Roberta Castrignanò, Tiziana Canestrelli, Daniele Sci Data Data Descriptor Understanding the genomic underpinnings of antipredatory behaviors is a hot topic in eco-evolutionary research. Yellow-bellied toad of the genus Bombina are textbook examples of the deimatic display, a time-structured behavior aimed at startling predators. Here, we generated the first de novo brain transcriptome of the Apennine yellow-bellied toad Bombina pachypus, a species showing inter-individual variation in the deimatic display. Through Rna-Seq experiments on a set of individuals showing distinct behavioral phenotypes, we generated 316,329,573 reads, which were assembled and annotated. The high-quality assembly was confirmed by assembly validators and by aligning the contigs against the de novo transcriptome with a mapping percentage higher than 91.0%. The homology annotation with DIAMOND (blastx) led to 77,391 contigs annotated on Nr, Swiss Prot and TrEMBL, whereas the domain and site protein prediction made with InterProScan led to 4747 GO-annotated and 1025 KEGG-annotated contigs. The B. pachypus transcriptome described here will be a valuable resource for further studies on the genomic underpinnings of behavioral variation in amphibians. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9561626/ /pubmed/36229462 http://dx.doi.org/10.1038/s41597-022-01724-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Data Descriptor Chiocchio, Andrea Libro, Pietro Martino, Giuseppe Bisconti, Roberta Castrignanò, Tiziana Canestrelli, Daniele Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title | Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title_full | Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title_fullStr | Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title_full_unstemmed | Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title_short | Brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
title_sort | brain de novo transcriptome assembly of a toad species showing polymorphic anti-predatory behavior |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561626/ https://www.ncbi.nlm.nih.gov/pubmed/36229462 http://dx.doi.org/10.1038/s41597-022-01724-5 |
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