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Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein

Intermittent compressive force influences human periodontal ligament (PDL) cell behavior that facilitates periodontal tissue regeneration. In response to mechanical stimuli, Yes-associated protein (YAP) has been recognized as a mechanosensitive transcriptional activator that regulates cell prolifera...

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Autores principales: Klincumhom, Nuttha, Lorthongpanich, Chanchao, Thumanu, Kanjana, Septham, Praphasri, Phomyu, Wutthikiat, Issaragrisil, Surapol, Pavasant, Prasit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561743/
https://www.ncbi.nlm.nih.gov/pubmed/36247165
http://dx.doi.org/10.1016/j.heliyon.2022.e10845
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author Klincumhom, Nuttha
Lorthongpanich, Chanchao
Thumanu, Kanjana
Septham, Praphasri
Phomyu, Wutthikiat
Issaragrisil, Surapol
Pavasant, Prasit
author_facet Klincumhom, Nuttha
Lorthongpanich, Chanchao
Thumanu, Kanjana
Septham, Praphasri
Phomyu, Wutthikiat
Issaragrisil, Surapol
Pavasant, Prasit
author_sort Klincumhom, Nuttha
collection PubMed
description Intermittent compressive force influences human periodontal ligament (PDL) cell behavior that facilitates periodontal tissue regeneration. In response to mechanical stimuli, Yes-associated protein (YAP) has been recognized as a mechanosensitive transcriptional activator that regulates cell proliferation and cell fate decisions. This study aimed to investigate whether compressive forces influence cell proliferation and cell fate decisions of human PDL cells via YAP signaling. YAP expression was silenced by shRNA. The effect of YAP on cell proliferation, adipogenesis and osteogenesis of PDL cells under ICF loading were determined. Adipogenic differentiation bias upon ICF loading was confirmed by fourier-transform infrared spectroscopy (FTIR). The results revealed that ICF-induced YAP promotes osteogenesis, but it inhibits adipogenesis in PDL cells. Depletion of YAP results in PDL cells that are irresponsive to ICF and, therefore, the failure of the PDL cells to undergo osteogenic differentiation. This was shown by a significant reduction in calcium deposited in the CF-derived osteoblasts of the YAP-knockdown (YAP-KD) PDL cells. As to control treatment, reduction of YAP promoted adipogenesis, whereas ICF-induced YAP inhibited this mechanism. However, the adipocyte differentiation in YAP-KD cells was not affected upon ICF treatment as the YAP-KD cells still exhibited a better adipogenic differentiation that was unrelated to the ICF. This study demonstrated that, in response to ICF treatment, YAP could be a crucial mechanosensitive transcriptional activator for the regulation of PDL cell behavior through a mechanobiological process. Our results may provide the possibility of facilitating PDL tissue regeneration by manipulation of the Hippo-YAP signaling pathway.
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spelling pubmed-95617432022-10-15 Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein Klincumhom, Nuttha Lorthongpanich, Chanchao Thumanu, Kanjana Septham, Praphasri Phomyu, Wutthikiat Issaragrisil, Surapol Pavasant, Prasit Heliyon Research Article Intermittent compressive force influences human periodontal ligament (PDL) cell behavior that facilitates periodontal tissue regeneration. In response to mechanical stimuli, Yes-associated protein (YAP) has been recognized as a mechanosensitive transcriptional activator that regulates cell proliferation and cell fate decisions. This study aimed to investigate whether compressive forces influence cell proliferation and cell fate decisions of human PDL cells via YAP signaling. YAP expression was silenced by shRNA. The effect of YAP on cell proliferation, adipogenesis and osteogenesis of PDL cells under ICF loading were determined. Adipogenic differentiation bias upon ICF loading was confirmed by fourier-transform infrared spectroscopy (FTIR). The results revealed that ICF-induced YAP promotes osteogenesis, but it inhibits adipogenesis in PDL cells. Depletion of YAP results in PDL cells that are irresponsive to ICF and, therefore, the failure of the PDL cells to undergo osteogenic differentiation. This was shown by a significant reduction in calcium deposited in the CF-derived osteoblasts of the YAP-knockdown (YAP-KD) PDL cells. As to control treatment, reduction of YAP promoted adipogenesis, whereas ICF-induced YAP inhibited this mechanism. However, the adipocyte differentiation in YAP-KD cells was not affected upon ICF treatment as the YAP-KD cells still exhibited a better adipogenic differentiation that was unrelated to the ICF. This study demonstrated that, in response to ICF treatment, YAP could be a crucial mechanosensitive transcriptional activator for the regulation of PDL cell behavior through a mechanobiological process. Our results may provide the possibility of facilitating PDL tissue regeneration by manipulation of the Hippo-YAP signaling pathway. Elsevier 2022-10-01 /pmc/articles/PMC9561743/ /pubmed/36247165 http://dx.doi.org/10.1016/j.heliyon.2022.e10845 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Klincumhom, Nuttha
Lorthongpanich, Chanchao
Thumanu, Kanjana
Septham, Praphasri
Phomyu, Wutthikiat
Issaragrisil, Surapol
Pavasant, Prasit
Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title_full Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title_fullStr Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title_full_unstemmed Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title_short Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
title_sort intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561743/
https://www.ncbi.nlm.nih.gov/pubmed/36247165
http://dx.doi.org/10.1016/j.heliyon.2022.e10845
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