Cargando…

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

It is critical to develop plant isoprenoid production when dealing with human-demanded industries such as flavoring, aroma, pigment, pharmaceuticals, and biomass used for biofuels. The methylerythritol phosphate (MEP) and mevalonic acid (MVA) plant pathways contribute to the dynamic production of is...

Descripción completa

Detalles Bibliográficos
Autores principales: Movahedi, Ali, Wei, Hui, Pucker, Boas, Ghaderi-Zefrehei, Mostafa, Rasouli, Fatemeh, Kiani-Pouya, Ali, Jiang, Tingbo, Zhuge, Qiang, Yang, Liming, Zhou, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562105/
https://www.ncbi.nlm.nih.gov/pubmed/36247639
http://dx.doi.org/10.3389/fpls.2022.968780
_version_ 1784808097309523968
author Movahedi, Ali
Wei, Hui
Pucker, Boas
Ghaderi-Zefrehei, Mostafa
Rasouli, Fatemeh
Kiani-Pouya, Ali
Jiang, Tingbo
Zhuge, Qiang
Yang, Liming
Zhou, Xiaohong
author_facet Movahedi, Ali
Wei, Hui
Pucker, Boas
Ghaderi-Zefrehei, Mostafa
Rasouli, Fatemeh
Kiani-Pouya, Ali
Jiang, Tingbo
Zhuge, Qiang
Yang, Liming
Zhou, Xiaohong
author_sort Movahedi, Ali
collection PubMed
description It is critical to develop plant isoprenoid production when dealing with human-demanded industries such as flavoring, aroma, pigment, pharmaceuticals, and biomass used for biofuels. The methylerythritol phosphate (MEP) and mevalonic acid (MVA) plant pathways contribute to the dynamic production of isoprenoid compounds. Still, the cross-talk between MVA and MEP in isoprenoid biosynthesis is not quite recognized. Regarding the rate-limiting steps in the MEP pathway through catalyzing 1-deoxy-D-xylulose5-phosphate synthase and 1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR) and also the rate-limiting step in the MVA pathway through catalyzing 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), the characterization and function of HMGR from Populus trichocarpa (PtHMGR) were analyzed. The results indicated that PtHMGR overexpressors (OEs) displayed various MEP and MVA-related gene expressions compared to NT poplars. The overexpression of PtDXR upregulated MEP-related genes and downregulated MVA-related genes. The overexpression of PtDXR and PtHMGR affected the isoprenoid production involved in both MVA and MEP pathways. Here, results illustrated that the PtHMGR and PtDXR play significant roles in regulating MEP and MVA-related genes and derived isoprenoids. This study clarifies cross-talk between MVA and MEP pathways. It demonstrates the key functions of HMGR and DXR in this cross-talk, which significantly contribute to regulate isoprenoid biosynthesis in poplars.
format Online
Article
Text
id pubmed-9562105
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95621052022-10-15 Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways Movahedi, Ali Wei, Hui Pucker, Boas Ghaderi-Zefrehei, Mostafa Rasouli, Fatemeh Kiani-Pouya, Ali Jiang, Tingbo Zhuge, Qiang Yang, Liming Zhou, Xiaohong Front Plant Sci Plant Science It is critical to develop plant isoprenoid production when dealing with human-demanded industries such as flavoring, aroma, pigment, pharmaceuticals, and biomass used for biofuels. The methylerythritol phosphate (MEP) and mevalonic acid (MVA) plant pathways contribute to the dynamic production of isoprenoid compounds. Still, the cross-talk between MVA and MEP in isoprenoid biosynthesis is not quite recognized. Regarding the rate-limiting steps in the MEP pathway through catalyzing 1-deoxy-D-xylulose5-phosphate synthase and 1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR) and also the rate-limiting step in the MVA pathway through catalyzing 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), the characterization and function of HMGR from Populus trichocarpa (PtHMGR) were analyzed. The results indicated that PtHMGR overexpressors (OEs) displayed various MEP and MVA-related gene expressions compared to NT poplars. The overexpression of PtDXR upregulated MEP-related genes and downregulated MVA-related genes. The overexpression of PtDXR and PtHMGR affected the isoprenoid production involved in both MVA and MEP pathways. Here, results illustrated that the PtHMGR and PtDXR play significant roles in regulating MEP and MVA-related genes and derived isoprenoids. This study clarifies cross-talk between MVA and MEP pathways. It demonstrates the key functions of HMGR and DXR in this cross-talk, which significantly contribute to regulate isoprenoid biosynthesis in poplars. Frontiers Media S.A. 2022-09-30 /pmc/articles/PMC9562105/ /pubmed/36247639 http://dx.doi.org/10.3389/fpls.2022.968780 Text en Copyright © 2022 Movahedi, Wei, Pucker, Ghaderi-Zefrehei, Rasouli, Kiani-Pouya, Jiang, Zhuge, Yang and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Movahedi, Ali
Wei, Hui
Pucker, Boas
Ghaderi-Zefrehei, Mostafa
Rasouli, Fatemeh
Kiani-Pouya, Ali
Jiang, Tingbo
Zhuge, Qiang
Yang, Liming
Zhou, Xiaohong
Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title_full Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title_fullStr Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title_full_unstemmed Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title_short Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
title_sort isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562105/
https://www.ncbi.nlm.nih.gov/pubmed/36247639
http://dx.doi.org/10.3389/fpls.2022.968780
work_keys_str_mv AT movahediali isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT weihui isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT puckerboas isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT ghaderizefreheimostafa isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT rasoulifatemeh isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT kianipouyaali isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT jiangtingbo isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT zhugeqiang isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT yangliming isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways
AT zhouxiaohong isoprenoidbiosynthesisregulationinpoplarsbymethylerythritolphosphateandmevalonicacidpathways