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Optical imaging detects metabolic signatures associated with oocyte quality(†)
Oocyte developmental potential is intimately linked to metabolism. Existing approaches to measure metabolism in the cumulus oocyte complex (COC) do not provide information on the separate cumulus and oocyte compartments. Development of an assay that achieves this may lead to an accurate diagnostic f...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562116/ https://www.ncbi.nlm.nih.gov/pubmed/35863764 http://dx.doi.org/10.1093/biolre/ioac145 |
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author | Tan, Tiffany C Y Brown, Hannah M Thompson, Jeremy G Mustafa, Sanam Dunning, Kylie R |
author_facet | Tan, Tiffany C Y Brown, Hannah M Thompson, Jeremy G Mustafa, Sanam Dunning, Kylie R |
author_sort | Tan, Tiffany C Y |
collection | PubMed |
description | Oocyte developmental potential is intimately linked to metabolism. Existing approaches to measure metabolism in the cumulus oocyte complex (COC) do not provide information on the separate cumulus and oocyte compartments. Development of an assay that achieves this may lead to an accurate diagnostic for oocyte quality. Optical imaging of the autofluorescent cofactors reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD) provides a spatially resolved indicator of metabolism via the optical redox ratio (FAD/[NAD(P)H + FAD]). This may provide an assessment of oocyte quality. Here, we determined whether the optical redox ratio is a robust methodology for measuring metabolism in the cumulus and oocyte compartments compared with oxygen consumption in the whole COC. We also determined whether optical imaging could detect metabolic differences associated with poor oocyte quality (etomoxir-treated). We used confocal microscopy to measure NAD(P)H and FAD, and extracellular flux to measure oxygen consumption. The optical redox ratio accurately reflected metabolism in the oocyte compartment when compared with oxygen consumption (whole COC). Etomoxir-treated COCs showed significantly lower levels of NAD(P)H and FAD compared to control. We further validated this approach using hyperspectral imaging, which is clinically compatible due to its low energy dose. This confirmed lower NAD(P)H and FAD in etomoxir-treated COCs. When comparing hyperspectral imaged vs non-imaged COCs, subsequent preimplantation development and post-transfer viability were comparable. Collectively, these results demonstrate that label-free optical imaging of metabolic cofactors is a safe and sensitive assay for measuring metabolism and has potential to assess oocyte developmental competence. |
format | Online Article Text |
id | pubmed-9562116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95621162022-10-18 Optical imaging detects metabolic signatures associated with oocyte quality(†) Tan, Tiffany C Y Brown, Hannah M Thompson, Jeremy G Mustafa, Sanam Dunning, Kylie R Biol Reprod Research Article Oocyte developmental potential is intimately linked to metabolism. Existing approaches to measure metabolism in the cumulus oocyte complex (COC) do not provide information on the separate cumulus and oocyte compartments. Development of an assay that achieves this may lead to an accurate diagnostic for oocyte quality. Optical imaging of the autofluorescent cofactors reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD) provides a spatially resolved indicator of metabolism via the optical redox ratio (FAD/[NAD(P)H + FAD]). This may provide an assessment of oocyte quality. Here, we determined whether the optical redox ratio is a robust methodology for measuring metabolism in the cumulus and oocyte compartments compared with oxygen consumption in the whole COC. We also determined whether optical imaging could detect metabolic differences associated with poor oocyte quality (etomoxir-treated). We used confocal microscopy to measure NAD(P)H and FAD, and extracellular flux to measure oxygen consumption. The optical redox ratio accurately reflected metabolism in the oocyte compartment when compared with oxygen consumption (whole COC). Etomoxir-treated COCs showed significantly lower levels of NAD(P)H and FAD compared to control. We further validated this approach using hyperspectral imaging, which is clinically compatible due to its low energy dose. This confirmed lower NAD(P)H and FAD in etomoxir-treated COCs. When comparing hyperspectral imaged vs non-imaged COCs, subsequent preimplantation development and post-transfer viability were comparable. Collectively, these results demonstrate that label-free optical imaging of metabolic cofactors is a safe and sensitive assay for measuring metabolism and has potential to assess oocyte developmental competence. Oxford University Press 2022-07-21 /pmc/articles/PMC9562116/ /pubmed/35863764 http://dx.doi.org/10.1093/biolre/ioac145 Text en © The Author(s) 2022. Published by Oxford University Press behalf of Society for the Study of Reproduction. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tan, Tiffany C Y Brown, Hannah M Thompson, Jeremy G Mustafa, Sanam Dunning, Kylie R Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title | Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title_full | Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title_fullStr | Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title_full_unstemmed | Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title_short | Optical imaging detects metabolic signatures associated with oocyte quality(†) |
title_sort | optical imaging detects metabolic signatures associated with oocyte quality(†) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562116/ https://www.ncbi.nlm.nih.gov/pubmed/35863764 http://dx.doi.org/10.1093/biolre/ioac145 |
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