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Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera

Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis...

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Autores principales: Uthailak, Naphatsamon, Adisakwattana, Poom, Thiangtrongjit, Tipparat, Limpanont, Yanin, Chusongsang, Phiraphol, Chusongsang, Yupa, Tanasarnprasert, Kanthi, Reamtong, Onrapak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562170/
https://www.ncbi.nlm.nih.gov/pubmed/36227939
http://dx.doi.org/10.1371/journal.pone.0275992
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author Uthailak, Naphatsamon
Adisakwattana, Poom
Thiangtrongjit, Tipparat
Limpanont, Yanin
Chusongsang, Phiraphol
Chusongsang, Yupa
Tanasarnprasert, Kanthi
Reamtong, Onrapak
author_facet Uthailak, Naphatsamon
Adisakwattana, Poom
Thiangtrongjit, Tipparat
Limpanont, Yanin
Chusongsang, Phiraphol
Chusongsang, Yupa
Tanasarnprasert, Kanthi
Reamtong, Onrapak
author_sort Uthailak, Naphatsamon
collection PubMed
description Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for S. mekongi, additional biomarkers are required. In this study, serum biomarkers associated with S. mekongi-infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of S. mekongi in mouse sera were analyzed using mass spectrometry-based proteomics. Serine protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of S. mekongi were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of S. mekongi was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development.
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spelling pubmed-95621702022-10-15 Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera Uthailak, Naphatsamon Adisakwattana, Poom Thiangtrongjit, Tipparat Limpanont, Yanin Chusongsang, Phiraphol Chusongsang, Yupa Tanasarnprasert, Kanthi Reamtong, Onrapak PLoS One Research Article Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for S. mekongi, additional biomarkers are required. In this study, serum biomarkers associated with S. mekongi-infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of S. mekongi in mouse sera were analyzed using mass spectrometry-based proteomics. Serine protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of S. mekongi were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of S. mekongi was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development. Public Library of Science 2022-10-13 /pmc/articles/PMC9562170/ /pubmed/36227939 http://dx.doi.org/10.1371/journal.pone.0275992 Text en © 2022 Uthailak et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Uthailak, Naphatsamon
Adisakwattana, Poom
Thiangtrongjit, Tipparat
Limpanont, Yanin
Chusongsang, Phiraphol
Chusongsang, Yupa
Tanasarnprasert, Kanthi
Reamtong, Onrapak
Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title_full Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title_fullStr Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title_full_unstemmed Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title_short Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
title_sort discovery of schistosoma mekongi circulating proteins and antigens in infected mouse sera
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562170/
https://www.ncbi.nlm.nih.gov/pubmed/36227939
http://dx.doi.org/10.1371/journal.pone.0275992
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