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Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562170/ https://www.ncbi.nlm.nih.gov/pubmed/36227939 http://dx.doi.org/10.1371/journal.pone.0275992 |
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author | Uthailak, Naphatsamon Adisakwattana, Poom Thiangtrongjit, Tipparat Limpanont, Yanin Chusongsang, Phiraphol Chusongsang, Yupa Tanasarnprasert, Kanthi Reamtong, Onrapak |
author_facet | Uthailak, Naphatsamon Adisakwattana, Poom Thiangtrongjit, Tipparat Limpanont, Yanin Chusongsang, Phiraphol Chusongsang, Yupa Tanasarnprasert, Kanthi Reamtong, Onrapak |
author_sort | Uthailak, Naphatsamon |
collection | PubMed |
description | Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for S. mekongi, additional biomarkers are required. In this study, serum biomarkers associated with S. mekongi-infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of S. mekongi in mouse sera were analyzed using mass spectrometry-based proteomics. Serine protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of S. mekongi were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of S. mekongi was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development. |
format | Online Article Text |
id | pubmed-9562170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95621702022-10-15 Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera Uthailak, Naphatsamon Adisakwattana, Poom Thiangtrongjit, Tipparat Limpanont, Yanin Chusongsang, Phiraphol Chusongsang, Yupa Tanasarnprasert, Kanthi Reamtong, Onrapak PLoS One Research Article Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for S. mekongi, additional biomarkers are required. In this study, serum biomarkers associated with S. mekongi-infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of S. mekongi in mouse sera were analyzed using mass spectrometry-based proteomics. Serine protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of S. mekongi were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of S. mekongi was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development. Public Library of Science 2022-10-13 /pmc/articles/PMC9562170/ /pubmed/36227939 http://dx.doi.org/10.1371/journal.pone.0275992 Text en © 2022 Uthailak et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Uthailak, Naphatsamon Adisakwattana, Poom Thiangtrongjit, Tipparat Limpanont, Yanin Chusongsang, Phiraphol Chusongsang, Yupa Tanasarnprasert, Kanthi Reamtong, Onrapak Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title | Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title_full | Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title_fullStr | Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title_full_unstemmed | Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title_short | Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera |
title_sort | discovery of schistosoma mekongi circulating proteins and antigens in infected mouse sera |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562170/ https://www.ncbi.nlm.nih.gov/pubmed/36227939 http://dx.doi.org/10.1371/journal.pone.0275992 |
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