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Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii

Plants and other photosynthetic organisms have been suggested as potential pervasive biosensors for nuclear nonproliferation monitoring. We demonstrate that ultrafast laser filament-induced fluorescence of chlorophyll in the green alga Chlamydomonas reinhardtii is a promising method for remote, in-f...

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Autores principales: Finney, Lauren A., Skrodzki, Patrick J., Peskosky, Nicholas, Burger, Milos, Nees, John, Krushelnick, Karl, Jovanovic, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562223/
https://www.ncbi.nlm.nih.gov/pubmed/36229516
http://dx.doi.org/10.1038/s41598-022-21404-z
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author Finney, Lauren A.
Skrodzki, Patrick J.
Peskosky, Nicholas
Burger, Milos
Nees, John
Krushelnick, Karl
Jovanovic, Igor
author_facet Finney, Lauren A.
Skrodzki, Patrick J.
Peskosky, Nicholas
Burger, Milos
Nees, John
Krushelnick, Karl
Jovanovic, Igor
author_sort Finney, Lauren A.
collection PubMed
description Plants and other photosynthetic organisms have been suggested as potential pervasive biosensors for nuclear nonproliferation monitoring. We demonstrate that ultrafast laser filament-induced fluorescence of chlorophyll in the green alga Chlamydomonas reinhardtii is a promising method for remote, in-field detection of stress from exposure to nuclear materials. This method holds an advantage over broad-area surveillance, such as solar-induced fluorescence monitoring, when targeting excitation of a specific plant would improve the detectability, for example when local biota density is low. After exposing C. reinhardtii to uranium, we find that the concentration of chlorophyll a, chlorophyll fluorescence lifetime, and carotenoid content increase. The increased fluorescence lifetime signifies a decrease in non-photochemical quenching. The simultaneous increase in carotenoid content implies oxidative stress, further confirmed by the production of radical oxygen species evidence in the steady-state absorption spectrum. This is potentially a unique signature of uranium, as previous work finds that heavy metal stress generally increases non-photochemical quenching. We identify the temporal profile of the chlorophyll fluorescence to be a distinguishing feature between uranium-exposed and unexposed algae. Discrimination of uranium-exposed samples is possible at a distance of [Formula: see text] 35 m with a single laser shot and a modest collection system, as determined through a combination of experiment and simulation of distance-scaled uncertainty in discriminating the temporal profiles. Illustrating the potential for remote detection, detection over 125 m would require 100 laser shots, commensurate with the detection time on the order of 1 s.
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spelling pubmed-95622232022-10-15 Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii Finney, Lauren A. Skrodzki, Patrick J. Peskosky, Nicholas Burger, Milos Nees, John Krushelnick, Karl Jovanovic, Igor Sci Rep Article Plants and other photosynthetic organisms have been suggested as potential pervasive biosensors for nuclear nonproliferation monitoring. We demonstrate that ultrafast laser filament-induced fluorescence of chlorophyll in the green alga Chlamydomonas reinhardtii is a promising method for remote, in-field detection of stress from exposure to nuclear materials. This method holds an advantage over broad-area surveillance, such as solar-induced fluorescence monitoring, when targeting excitation of a specific plant would improve the detectability, for example when local biota density is low. After exposing C. reinhardtii to uranium, we find that the concentration of chlorophyll a, chlorophyll fluorescence lifetime, and carotenoid content increase. The increased fluorescence lifetime signifies a decrease in non-photochemical quenching. The simultaneous increase in carotenoid content implies oxidative stress, further confirmed by the production of radical oxygen species evidence in the steady-state absorption spectrum. This is potentially a unique signature of uranium, as previous work finds that heavy metal stress generally increases non-photochemical quenching. We identify the temporal profile of the chlorophyll fluorescence to be a distinguishing feature between uranium-exposed and unexposed algae. Discrimination of uranium-exposed samples is possible at a distance of [Formula: see text] 35 m with a single laser shot and a modest collection system, as determined through a combination of experiment and simulation of distance-scaled uncertainty in discriminating the temporal profiles. Illustrating the potential for remote detection, detection over 125 m would require 100 laser shots, commensurate with the detection time on the order of 1 s. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9562223/ /pubmed/36229516 http://dx.doi.org/10.1038/s41598-022-21404-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Finney, Lauren A.
Skrodzki, Patrick J.
Peskosky, Nicholas
Burger, Milos
Nees, John
Krushelnick, Karl
Jovanovic, Igor
Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title_full Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title_fullStr Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title_full_unstemmed Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title_short Ultrafast laser filament-induced fluorescence for detecting uranium stress in Chlamydomonas reinhardtii
title_sort ultrafast laser filament-induced fluorescence for detecting uranium stress in chlamydomonas reinhardtii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562223/
https://www.ncbi.nlm.nih.gov/pubmed/36229516
http://dx.doi.org/10.1038/s41598-022-21404-z
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