Cargando…

Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies

Fetal growth restriction (FGR) is associated with short- and long-term morbidity, often with fetal compromise in utero, evidenced by abnormal Doppler velocimetry of fetal vessels. Lipids are vital for growth and development, but metabolism in FGR pregnancy, where fetuses do not grow to full genetic...

Descripción completa

Detalles Bibliográficos
Autores principales: Chassen, Stephanie S., Zemski-Berry, Karin, Raymond-Whish, Stefanie, Driver, Camille, Hobbins, John C., Powell, Theresa L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562243/
https://www.ncbi.nlm.nih.gov/pubmed/36231072
http://dx.doi.org/10.3390/cells11193110
_version_ 1784808127733956608
author Chassen, Stephanie S.
Zemski-Berry, Karin
Raymond-Whish, Stefanie
Driver, Camille
Hobbins, John C.
Powell, Theresa L.
author_facet Chassen, Stephanie S.
Zemski-Berry, Karin
Raymond-Whish, Stefanie
Driver, Camille
Hobbins, John C.
Powell, Theresa L.
author_sort Chassen, Stephanie S.
collection PubMed
description Fetal growth restriction (FGR) is associated with short- and long-term morbidity, often with fetal compromise in utero, evidenced by abnormal Doppler velocimetry of fetal vessels. Lipids are vital for growth and development, but metabolism in FGR pregnancy, where fetuses do not grow to full genetic potential, is poorly understood. We hypothesize that triglyceride concentrations are increased in placentas and that important complex lipids are reduced in cord plasma from pregnancies producing the smallest babies (birth weight < 5%) and correlate with ultrasound Dopplers. Dopplers (umbilical artery, UA; middle cerebral artery, MCA) were assessed longitudinally in pregnancies diagnosed with estimated fetal weight (EFW) < 10% at ≥29 weeks gestation. For a subset of enrolled women, placentas and cord blood were collected at delivery, fatty acids were extracted and targeted lipid class analysis (triglyceride, TG; phosphatidylcholine, PC; lysophosphatidylcholine, LPC; eicosanoid) performed by LCMS. For this sub-analysis, participants were categorized as FGR (Fenton birth weight, BW ≤ 5%) or SGA “controls” (Fenton BW > 5%). FGRs (n = 8) delivered 1 week earlier (p = 0.04), were 29% smaller (p = 0.002), and had 133% higher UA pulsatility index (PI, p = 0.02) than SGAs (n = 12). FGR plasma TG, free arachidonic acid (AA), and several eicosanoids were increased (p < 0.05); docosahexaenoic acid (DHA)-LPC was decreased (p < 0.01). Plasma TG correlated inversely with BW (p < 0.05). Plasma EET, non-esterified AA, and DHA correlated inversely with BW and directly with UA PI (p < 0.05). Placental DHA-PC and AA-PC correlated directly with MCA PI (p < 0.05). In fetuses initially referred for inadequate fetal growth (EFW < 10%), those with BW ≤ 5% demonstrated distinctly different cord plasma lipid profiles than those with BW > 5%, which correlated with Doppler PIs. This provides new insights into fetal lipidomic response to the FGR in utero environment. The impact of these changes on specific processes of growth and development (particularly fetal brain) have not been elucidated, but the relationship with Doppler PI may provide additional context for FGR surveillance, and a more targeted approach to nutritional management of these infants.
format Online
Article
Text
id pubmed-9562243
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95622432022-10-15 Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies Chassen, Stephanie S. Zemski-Berry, Karin Raymond-Whish, Stefanie Driver, Camille Hobbins, John C. Powell, Theresa L. Cells Article Fetal growth restriction (FGR) is associated with short- and long-term morbidity, often with fetal compromise in utero, evidenced by abnormal Doppler velocimetry of fetal vessels. Lipids are vital for growth and development, but metabolism in FGR pregnancy, where fetuses do not grow to full genetic potential, is poorly understood. We hypothesize that triglyceride concentrations are increased in placentas and that important complex lipids are reduced in cord plasma from pregnancies producing the smallest babies (birth weight < 5%) and correlate with ultrasound Dopplers. Dopplers (umbilical artery, UA; middle cerebral artery, MCA) were assessed longitudinally in pregnancies diagnosed with estimated fetal weight (EFW) < 10% at ≥29 weeks gestation. For a subset of enrolled women, placentas and cord blood were collected at delivery, fatty acids were extracted and targeted lipid class analysis (triglyceride, TG; phosphatidylcholine, PC; lysophosphatidylcholine, LPC; eicosanoid) performed by LCMS. For this sub-analysis, participants were categorized as FGR (Fenton birth weight, BW ≤ 5%) or SGA “controls” (Fenton BW > 5%). FGRs (n = 8) delivered 1 week earlier (p = 0.04), were 29% smaller (p = 0.002), and had 133% higher UA pulsatility index (PI, p = 0.02) than SGAs (n = 12). FGR plasma TG, free arachidonic acid (AA), and several eicosanoids were increased (p < 0.05); docosahexaenoic acid (DHA)-LPC was decreased (p < 0.01). Plasma TG correlated inversely with BW (p < 0.05). Plasma EET, non-esterified AA, and DHA correlated inversely with BW and directly with UA PI (p < 0.05). Placental DHA-PC and AA-PC correlated directly with MCA PI (p < 0.05). In fetuses initially referred for inadequate fetal growth (EFW < 10%), those with BW ≤ 5% demonstrated distinctly different cord plasma lipid profiles than those with BW > 5%, which correlated with Doppler PIs. This provides new insights into fetal lipidomic response to the FGR in utero environment. The impact of these changes on specific processes of growth and development (particularly fetal brain) have not been elucidated, but the relationship with Doppler PI may provide additional context for FGR surveillance, and a more targeted approach to nutritional management of these infants. MDPI 2022-10-02 /pmc/articles/PMC9562243/ /pubmed/36231072 http://dx.doi.org/10.3390/cells11193110 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chassen, Stephanie S.
Zemski-Berry, Karin
Raymond-Whish, Stefanie
Driver, Camille
Hobbins, John C.
Powell, Theresa L.
Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title_full Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title_fullStr Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title_full_unstemmed Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title_short Altered Cord Blood Lipid Concentrations Correlate with Birth Weight and Doppler Velocimetry of Fetal Vessels in Human Fetal Growth Restriction Pregnancies
title_sort altered cord blood lipid concentrations correlate with birth weight and doppler velocimetry of fetal vessels in human fetal growth restriction pregnancies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562243/
https://www.ncbi.nlm.nih.gov/pubmed/36231072
http://dx.doi.org/10.3390/cells11193110
work_keys_str_mv AT chassenstephanies alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies
AT zemskiberrykarin alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies
AT raymondwhishstefanie alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies
AT drivercamille alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies
AT hobbinsjohnc alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies
AT powelltheresal alteredcordbloodlipidconcentrationscorrelatewithbirthweightanddopplervelocimetryoffetalvesselsinhumanfetalgrowthrestrictionpregnancies