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Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway

Zinc oxide-silver (ZnO–Ag), and zinc oxide-gold (ZnO–Au) nano-composites were prepared through wet chemical process and laced into single-walled carbon nanotubes (SWCNTs) to yield ZnO–Ag-SWCNTs, and ZnO–Au-SWCNTs hybrids. These nano-composite-laced SWCNTs hybrids were characterized using Raman spect...

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Autores principales: Al Rugaie, Osamah, Jabir, Majid S., Mohammed, Mustafa K. A., Abbas, Ruaa H., Ahmed, Duha S., Sulaiman, Ghassan M., Mohammed, Salman A. A., Khan, Riaz A., Al-Regaiey, Khalid A., Alsharidah, Mansour, Mohany, Khalid M., Mohammed, Hamdoon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562326/
https://www.ncbi.nlm.nih.gov/pubmed/36229515
http://dx.doi.org/10.1038/s41598-022-22193-1
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author Al Rugaie, Osamah
Jabir, Majid S.
Mohammed, Mustafa K. A.
Abbas, Ruaa H.
Ahmed, Duha S.
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Al-Regaiey, Khalid A.
Alsharidah, Mansour
Mohany, Khalid M.
Mohammed, Hamdoon A.
author_facet Al Rugaie, Osamah
Jabir, Majid S.
Mohammed, Mustafa K. A.
Abbas, Ruaa H.
Ahmed, Duha S.
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Al-Regaiey, Khalid A.
Alsharidah, Mansour
Mohany, Khalid M.
Mohammed, Hamdoon A.
author_sort Al Rugaie, Osamah
collection PubMed
description Zinc oxide-silver (ZnO–Ag), and zinc oxide-gold (ZnO–Au) nano-composites were prepared through wet chemical process and laced into single-walled carbon nanotubes (SWCNTs) to yield ZnO–Ag-SWCNTs, and ZnO–Au-SWCNTs hybrids. These nano-composite-laced SWCNTs hybrids were characterized using Raman spectroscopic, X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analyses. The hybrids were evaluated for their effects on phagocytic cells and bactericidal activity against the gram-negative bacteria E. coli. Their phagocytic cell activities and intracellular killing actions were found to be significantly increased, as the ZnO–Ag-SWCNTs and ZnO–Au-SWCNTs nano-hybrids induced widespread clearance of Escherichia coli. An increase in the production of reactive oxygen species (ROS) also led to upregulated phagocytosis, which was determined mechanistically to involve the phagocyte NADPH oxidase (NOX2) pathway. The findings emphasized the roles of ZnO–Ag- and ZnO–Au-decorated SWCNTs in the prevention of bacterial infection by inhibiting biofilm formation, showing the potential to be utilized as catheter coatings in the clinic.
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spelling pubmed-95623262022-10-15 Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway Al Rugaie, Osamah Jabir, Majid S. Mohammed, Mustafa K. A. Abbas, Ruaa H. Ahmed, Duha S. Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Al-Regaiey, Khalid A. Alsharidah, Mansour Mohany, Khalid M. Mohammed, Hamdoon A. Sci Rep Article Zinc oxide-silver (ZnO–Ag), and zinc oxide-gold (ZnO–Au) nano-composites were prepared through wet chemical process and laced into single-walled carbon nanotubes (SWCNTs) to yield ZnO–Ag-SWCNTs, and ZnO–Au-SWCNTs hybrids. These nano-composite-laced SWCNTs hybrids were characterized using Raman spectroscopic, X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analyses. The hybrids were evaluated for their effects on phagocytic cells and bactericidal activity against the gram-negative bacteria E. coli. Their phagocytic cell activities and intracellular killing actions were found to be significantly increased, as the ZnO–Ag-SWCNTs and ZnO–Au-SWCNTs nano-hybrids induced widespread clearance of Escherichia coli. An increase in the production of reactive oxygen species (ROS) also led to upregulated phagocytosis, which was determined mechanistically to involve the phagocyte NADPH oxidase (NOX2) pathway. The findings emphasized the roles of ZnO–Ag- and ZnO–Au-decorated SWCNTs in the prevention of bacterial infection by inhibiting biofilm formation, showing the potential to be utilized as catheter coatings in the clinic. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9562326/ /pubmed/36229515 http://dx.doi.org/10.1038/s41598-022-22193-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Al Rugaie, Osamah
Jabir, Majid S.
Mohammed, Mustafa K. A.
Abbas, Ruaa H.
Ahmed, Duha S.
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Al-Regaiey, Khalid A.
Alsharidah, Mansour
Mohany, Khalid M.
Mohammed, Hamdoon A.
Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title_full Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title_fullStr Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title_full_unstemmed Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title_short Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway
title_sort modification of swcnts with hybrid materials zno–ag and zno–au for enhancing bactericidal activity of phagocytic cells against escherichia coli through nox2 pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562326/
https://www.ncbi.nlm.nih.gov/pubmed/36229515
http://dx.doi.org/10.1038/s41598-022-22193-1
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