Protocol for electrical modulation of ERK signaling dynamics in cell lines

Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real...

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Detalles Bibliográficos
Autores principales: Zhu, Kan, Guo, Liang, Patel, Preena, Albeck, John, Qing, Quan, Losert, Wolfgang, Zhao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562398/
https://www.ncbi.nlm.nih.gov/pubmed/36208452
http://dx.doi.org/10.1016/j.xpro.2022.101752
Descripción
Sumario:Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

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