Protocol for electrical modulation of ERK signaling dynamics in cell lines

Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real...

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Autores principales: Zhu, Kan, Guo, Liang, Patel, Preena, Albeck, John, Qing, Quan, Losert, Wolfgang, Zhao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562398/
https://www.ncbi.nlm.nih.gov/pubmed/36208452
http://dx.doi.org/10.1016/j.xpro.2022.101752
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author Zhu, Kan
Guo, Liang
Patel, Preena
Albeck, John
Qing, Quan
Losert, Wolfgang
Zhao, Min
author_facet Zhu, Kan
Guo, Liang
Patel, Preena
Albeck, John
Qing, Quan
Losert, Wolfgang
Zhao, Min
author_sort Zhu, Kan
collection PubMed
description Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).
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spelling pubmed-95623982022-10-15 Protocol for electrical modulation of ERK signaling dynamics in cell lines Zhu, Kan Guo, Liang Patel, Preena Albeck, John Qing, Quan Losert, Wolfgang Zhao, Min STAR Protoc Protocol Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021). Elsevier 2022-10-07 /pmc/articles/PMC9562398/ /pubmed/36208452 http://dx.doi.org/10.1016/j.xpro.2022.101752 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhu, Kan
Guo, Liang
Patel, Preena
Albeck, John
Qing, Quan
Losert, Wolfgang
Zhao, Min
Protocol for electrical modulation of ERK signaling dynamics in cell lines
title Protocol for electrical modulation of ERK signaling dynamics in cell lines
title_full Protocol for electrical modulation of ERK signaling dynamics in cell lines
title_fullStr Protocol for electrical modulation of ERK signaling dynamics in cell lines
title_full_unstemmed Protocol for electrical modulation of ERK signaling dynamics in cell lines
title_short Protocol for electrical modulation of ERK signaling dynamics in cell lines
title_sort protocol for electrical modulation of erk signaling dynamics in cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562398/
https://www.ncbi.nlm.nih.gov/pubmed/36208452
http://dx.doi.org/10.1016/j.xpro.2022.101752
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