Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system

This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissu...

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Detalles Bibliográficos
Autores principales: Wolf, Julian, Sabage, Luís E., Sun, Young Joo, Mahajan, Vinit B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562426/
https://www.ncbi.nlm.nih.gov/pubmed/36208453
http://dx.doi.org/10.1016/j.xpro.2022.101754
Descripción
Sumario:This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression.

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