Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system

This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissu...

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Detalles Bibliográficos
Autores principales: Wolf, Julian, Sabage, Luís E., Sun, Young Joo, Mahajan, Vinit B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562426/
https://www.ncbi.nlm.nih.gov/pubmed/36208453
http://dx.doi.org/10.1016/j.xpro.2022.101754
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author Wolf, Julian
Sabage, Luís E.
Sun, Young Joo
Mahajan, Vinit B.
author_facet Wolf, Julian
Sabage, Luís E.
Sun, Young Joo
Mahajan, Vinit B.
author_sort Wolf, Julian
collection PubMed
description This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression.
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spelling pubmed-95624262022-10-15 Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system Wolf, Julian Sabage, Luís E. Sun, Young Joo Mahajan, Vinit B. STAR Protoc Protocol This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression. Elsevier 2022-10-07 /pmc/articles/PMC9562426/ /pubmed/36208453 http://dx.doi.org/10.1016/j.xpro.2022.101754 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wolf, Julian
Sabage, Luís E.
Sun, Young Joo
Mahajan, Vinit B.
Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title_full Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title_fullStr Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title_full_unstemmed Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title_short Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
title_sort protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562426/
https://www.ncbi.nlm.nih.gov/pubmed/36208453
http://dx.doi.org/10.1016/j.xpro.2022.101754
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