Protocol for renal ischemia-reperfusion injury by flank incisions in mice

Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier wi...

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Detalles Bibliográficos
Autores principales: Cheng, Yung-Ting, Tu, Ya-Chun, Chou, Yu-Hsiang, Lai, Chun-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562430/
https://www.ncbi.nlm.nih.gov/pubmed/36208451
http://dx.doi.org/10.1016/j.xpro.2022.101678
Descripción
Sumario:Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014).

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