Protocol for renal ischemia-reperfusion injury by flank incisions in mice

Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier wi...

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Autores principales: Cheng, Yung-Ting, Tu, Ya-Chun, Chou, Yu-Hsiang, Lai, Chun-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562430/
https://www.ncbi.nlm.nih.gov/pubmed/36208451
http://dx.doi.org/10.1016/j.xpro.2022.101678
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author Cheng, Yung-Ting
Tu, Ya-Chun
Chou, Yu-Hsiang
Lai, Chun-Fu
author_facet Cheng, Yung-Ting
Tu, Ya-Chun
Chou, Yu-Hsiang
Lai, Chun-Fu
author_sort Cheng, Yung-Ting
collection PubMed
description Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014).
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spelling pubmed-95624302022-10-15 Protocol for renal ischemia-reperfusion injury by flank incisions in mice Cheng, Yung-Ting Tu, Ya-Chun Chou, Yu-Hsiang Lai, Chun-Fu STAR Protoc Protocol Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014). Elsevier 2022-10-07 /pmc/articles/PMC9562430/ /pubmed/36208451 http://dx.doi.org/10.1016/j.xpro.2022.101678 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Cheng, Yung-Ting
Tu, Ya-Chun
Chou, Yu-Hsiang
Lai, Chun-Fu
Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title_full Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title_fullStr Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title_full_unstemmed Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title_short Protocol for renal ischemia-reperfusion injury by flank incisions in mice
title_sort protocol for renal ischemia-reperfusion injury by flank incisions in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562430/
https://www.ncbi.nlm.nih.gov/pubmed/36208451
http://dx.doi.org/10.1016/j.xpro.2022.101678
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