Cargando…

Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula

Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (...

Descripción completa

Detalles Bibliográficos
Autores principales: Ribeiro, Bianca, Erffelinck, Marie-Laure, Lacchini, Elia, Ceulemans, Evi, Colinas, Maite, Williams, Clara, Van Hamme, Evelien, De Clercq, Rebecca, Perassolo, Maria, Goossens, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562455/
https://www.ncbi.nlm.nih.gov/pubmed/36247618
http://dx.doi.org/10.3389/fpls.2022.903793
_version_ 1784808176312385536
author Ribeiro, Bianca
Erffelinck, Marie-Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
author_facet Ribeiro, Bianca
Erffelinck, Marie-Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
author_sort Ribeiro, Bianca
collection PubMed
description Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.
format Online
Article
Text
id pubmed-9562455
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95624552022-10-15 Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula Ribeiro, Bianca Erffelinck, Marie-Laure Lacchini, Elia Ceulemans, Evi Colinas, Maite Williams, Clara Van Hamme, Evelien De Clercq, Rebecca Perassolo, Maria Goossens, Alain Front Plant Sci Plant Science Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus. Frontiers Media S.A. 2022-09-30 /pmc/articles/PMC9562455/ /pubmed/36247618 http://dx.doi.org/10.3389/fpls.2022.903793 Text en Copyright © 2022 Ribeiro, Erffelinck, Lacchini, Ceulemans, Colinas, Williams, Van Hamme, De Clercq, Perassolo and Goossens https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ribeiro, Bianca
Erffelinck, Marie-Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_full Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_fullStr Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_full_unstemmed Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_short Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_sort interference between er stress-related bzip-type and jasmonate-inducible bhlh-type transcription factors in the regulation of triterpene saponin biosynthesis in medicago truncatula
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562455/
https://www.ncbi.nlm.nih.gov/pubmed/36247618
http://dx.doi.org/10.3389/fpls.2022.903793
work_keys_str_mv AT ribeirobianca interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT erffelinckmarielaure interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT lacchinielia interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT ceulemansevi interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT colinasmaite interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT williamsclara interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT vanhammeevelien interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT declercqrebecca interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT perassolomaria interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula
AT goossensalain interferencebetweenerstressrelatedbziptypeandjasmonateinduciblebhlhtypetranscriptionfactorsintheregulationoftriterpenesaponinbiosynthesisinmedicagotruncatula