Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells

Regulatory T cells (Tregs) can inhibit the occurrence of autoimmune diseases and increase the activation threshold of the immune response. Here, we present schemes for the isolation and culture of natural human regulatory T cells (nTregs) and in vitro-induced Tregs (iTregs). Appropriate concentratio...

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Detalles Bibliográficos
Autores principales: Gu, Jian, Shao, Qing, Zhou, Jinren, Chen, Qiuyang, Lu, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563191/
https://www.ncbi.nlm.nih.gov/pubmed/36219560
http://dx.doi.org/10.1016/j.xpro.2022.101740
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author Gu, Jian
Shao, Qing
Zhou, Jinren
Chen, Qiuyang
Lu, Ling
author_facet Gu, Jian
Shao, Qing
Zhou, Jinren
Chen, Qiuyang
Lu, Ling
author_sort Gu, Jian
collection PubMed
description Regulatory T cells (Tregs) can inhibit the occurrence of autoimmune diseases and increase the activation threshold of the immune response. Here, we present schemes for the isolation and culture of natural human regulatory T cells (nTregs) and in vitro-induced Tregs (iTregs). Appropriate concentrations of TGF-β, IL-2, retinoic acid (atRA), and rapamycin were used to promote proliferation to meet sample needs in basic research, especially in technologies such as sequencing. For complete details on the use and execution of this protocol, please refer to Lu et al. (2014a) and Gu et al. (2022).
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spelling pubmed-95631912022-10-15 Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells Gu, Jian Shao, Qing Zhou, Jinren Chen, Qiuyang Lu, Ling STAR Protoc Protocol Regulatory T cells (Tregs) can inhibit the occurrence of autoimmune diseases and increase the activation threshold of the immune response. Here, we present schemes for the isolation and culture of natural human regulatory T cells (nTregs) and in vitro-induced Tregs (iTregs). Appropriate concentrations of TGF-β, IL-2, retinoic acid (atRA), and rapamycin were used to promote proliferation to meet sample needs in basic research, especially in technologies such as sequencing. For complete details on the use and execution of this protocol, please refer to Lu et al. (2014a) and Gu et al. (2022). Elsevier 2022-10-09 /pmc/articles/PMC9563191/ /pubmed/36219560 http://dx.doi.org/10.1016/j.xpro.2022.101740 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gu, Jian
Shao, Qing
Zhou, Jinren
Chen, Qiuyang
Lu, Ling
Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title_full Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title_fullStr Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title_full_unstemmed Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title_short Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
title_sort protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory t cells and induced regulatory t cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563191/
https://www.ncbi.nlm.nih.gov/pubmed/36219560
http://dx.doi.org/10.1016/j.xpro.2022.101740
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