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Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management
African swine fever (ASF) is a hemorrhagic and often fatal disease occurring in domestic pigs and wild boars. ASF can potentially greatly impact the global trade of pigs and pork products and threaten global food security. Outbreaks of ASF must be notified to the World Organization for Animal Health...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563241/ https://www.ncbi.nlm.nih.gov/pubmed/36246220 http://dx.doi.org/10.3389/fmicb.2022.1025758 |
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author | Zeng, Dexin Qian, Bingxu Li, Yunfei Zong, Kai Peng, Wanqing Liao, Kai Yu, Xiaofeng Sun, Juanjuan Lv, Xiaying Ding, Liu Wang, Manman Zhou, Tingting Jiang, Yuan Li, Jinming Xue, Feng Wu, Xiaodong Dai, Jianjun |
author_facet | Zeng, Dexin Qian, Bingxu Li, Yunfei Zong, Kai Peng, Wanqing Liao, Kai Yu, Xiaofeng Sun, Juanjuan Lv, Xiaying Ding, Liu Wang, Manman Zhou, Tingting Jiang, Yuan Li, Jinming Xue, Feng Wu, Xiaodong Dai, Jianjun |
author_sort | Zeng, Dexin |
collection | PubMed |
description | African swine fever (ASF) is a hemorrhagic and often fatal disease occurring in domestic pigs and wild boars. ASF can potentially greatly impact the global trade of pigs and pork products and threaten global food security. Outbreaks of ASF must be notified to the World Organization for Animal Health. In this study, we analyzed the feasibility of applying propidium monoazide (PMA) pretreatment-based infectious virus detection technology to ASF prevention and control and investigated the prospects of applying this technology for epidemic monitoring, disinfection effect evaluation, and drug development. PMA as a nucleic acid dye can enter damaged cells and undergo irreversible covalent crosslinking with nucleic acid under halogen light to prevent its amplification. Although this technology has been widely used for the rapid detection of viable bacteria, its application in viruses is rare. Therefore, we analyzed the theoretical feasibility of applying this technology to the African swine fever virus (ASFV) in terms of gene and cell composition. Rapid infectious ASFV detection technology based on PMA pretreatment would greatly enhance all aspects of ASF prevention and control, such as epidemic monitoring, disinfection treatment, and drug development. The introduction of this technology will also greatly improve the ability to prevent and control ASF. |
format | Online Article Text |
id | pubmed-9563241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95632412022-10-15 Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management Zeng, Dexin Qian, Bingxu Li, Yunfei Zong, Kai Peng, Wanqing Liao, Kai Yu, Xiaofeng Sun, Juanjuan Lv, Xiaying Ding, Liu Wang, Manman Zhou, Tingting Jiang, Yuan Li, Jinming Xue, Feng Wu, Xiaodong Dai, Jianjun Front Microbiol Microbiology African swine fever (ASF) is a hemorrhagic and often fatal disease occurring in domestic pigs and wild boars. ASF can potentially greatly impact the global trade of pigs and pork products and threaten global food security. Outbreaks of ASF must be notified to the World Organization for Animal Health. In this study, we analyzed the feasibility of applying propidium monoazide (PMA) pretreatment-based infectious virus detection technology to ASF prevention and control and investigated the prospects of applying this technology for epidemic monitoring, disinfection effect evaluation, and drug development. PMA as a nucleic acid dye can enter damaged cells and undergo irreversible covalent crosslinking with nucleic acid under halogen light to prevent its amplification. Although this technology has been widely used for the rapid detection of viable bacteria, its application in viruses is rare. Therefore, we analyzed the theoretical feasibility of applying this technology to the African swine fever virus (ASFV) in terms of gene and cell composition. Rapid infectious ASFV detection technology based on PMA pretreatment would greatly enhance all aspects of ASF prevention and control, such as epidemic monitoring, disinfection treatment, and drug development. The introduction of this technology will also greatly improve the ability to prevent and control ASF. Frontiers Media S.A. 2022-09-30 /pmc/articles/PMC9563241/ /pubmed/36246220 http://dx.doi.org/10.3389/fmicb.2022.1025758 Text en Copyright © 2022 Zeng, Qian, Li, Zong, Peng, Liao, Yu, Sun, Lv, Ding, Wang, Zhou, Jiang, Li, Xue, Wu and Dai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zeng, Dexin Qian, Bingxu Li, Yunfei Zong, Kai Peng, Wanqing Liao, Kai Yu, Xiaofeng Sun, Juanjuan Lv, Xiaying Ding, Liu Wang, Manman Zhou, Tingting Jiang, Yuan Li, Jinming Xue, Feng Wu, Xiaodong Dai, Jianjun Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title | Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title_full | Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title_fullStr | Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title_full_unstemmed | Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title_short | Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management |
title_sort | prospects for the application of infectious virus detection technology based on propidium monoazide in african swine fever management |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563241/ https://www.ncbi.nlm.nih.gov/pubmed/36246220 http://dx.doi.org/10.3389/fmicb.2022.1025758 |
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