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Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions

The success of dental implants lies in their strong and lasting integration into the patient’s receiving bone. The first biological interactions at the implant surface determine the subsequent evolution of the integration process. In this study we set our objective to analyze the mechanistic interac...

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Autores principales: Anitua, Eduardo, Tejero, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563433/
https://www.ncbi.nlm.nih.gov/pubmed/36231011
http://dx.doi.org/10.3390/cells11193048
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author Anitua, Eduardo
Tejero, Ricardo
author_facet Anitua, Eduardo
Tejero, Ricardo
author_sort Anitua, Eduardo
collection PubMed
description The success of dental implants lies in their strong and lasting integration into the patient’s receiving bone. The first biological interactions at the implant surface determine the subsequent evolution of the integration process. In this study we set our objective to analyze the mechanistic interaction of the early regenerative matrix at implant surfaces modified with calcium ions (Ca) as compared to standard implant surfaces (NoCa). We put the surfaces in a Quartz Crystal Microbalance with Dissipation (QCM-D) to monitor the frequency shift (f) and the viscoelastic properties of the adsorbed biofilms and used Scanning Electron Microscopy (SEM) to visualize the resulting interfaces. Upon the addition of human blood plasma, Ca surfaces formed an adsorbed three-dimensional film attached to the surface (∆f = −40 Hz), while with NoCa, the biofilm formed but was not attached to the surface (∆f = 0 Hz). After 20 min in blood, two representative commercial implants with Ca and NoCa surfaces showed also distinct interfaces: Ca implants formed a visible clot attached to the implant which was composed mainly of platelets (Surface Coverage: 40 ± 20%) and some red blood cells (SC: 9 ± 3%) entrapped within a fibrin network (SC: 93 ± 5%). The NoCa implants were largely populated by red blood cells (SC: 67 ± 12%) with scarce fibrin remnants (SC: 3 ± 2%), and the implants showed no clot on their surfaces macroscopically. The pre-clinical and clinical results discussed in this work encourage the modification of titanium implant surfaces with calcium ions to improve the bone regenerative process. Taken together, these results add more information about the roles of Ca ions in bridging the formation of the provisional matrix at implant surfaces and their effects on implant osseointegration.
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spelling pubmed-95634332022-10-15 Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions Anitua, Eduardo Tejero, Ricardo Cells Article The success of dental implants lies in their strong and lasting integration into the patient’s receiving bone. The first biological interactions at the implant surface determine the subsequent evolution of the integration process. In this study we set our objective to analyze the mechanistic interaction of the early regenerative matrix at implant surfaces modified with calcium ions (Ca) as compared to standard implant surfaces (NoCa). We put the surfaces in a Quartz Crystal Microbalance with Dissipation (QCM-D) to monitor the frequency shift (f) and the viscoelastic properties of the adsorbed biofilms and used Scanning Electron Microscopy (SEM) to visualize the resulting interfaces. Upon the addition of human blood plasma, Ca surfaces formed an adsorbed three-dimensional film attached to the surface (∆f = −40 Hz), while with NoCa, the biofilm formed but was not attached to the surface (∆f = 0 Hz). After 20 min in blood, two representative commercial implants with Ca and NoCa surfaces showed also distinct interfaces: Ca implants formed a visible clot attached to the implant which was composed mainly of platelets (Surface Coverage: 40 ± 20%) and some red blood cells (SC: 9 ± 3%) entrapped within a fibrin network (SC: 93 ± 5%). The NoCa implants were largely populated by red blood cells (SC: 67 ± 12%) with scarce fibrin remnants (SC: 3 ± 2%), and the implants showed no clot on their surfaces macroscopically. The pre-clinical and clinical results discussed in this work encourage the modification of titanium implant surfaces with calcium ions to improve the bone regenerative process. Taken together, these results add more information about the roles of Ca ions in bridging the formation of the provisional matrix at implant surfaces and their effects on implant osseointegration. MDPI 2022-09-29 /pmc/articles/PMC9563433/ /pubmed/36231011 http://dx.doi.org/10.3390/cells11193048 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Anitua, Eduardo
Tejero, Ricardo
Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title_full Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title_fullStr Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title_full_unstemmed Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title_short Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
title_sort provisional matrix formation at implant surfaces—the bridging role of calcium ions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563433/
https://www.ncbi.nlm.nih.gov/pubmed/36231011
http://dx.doi.org/10.3390/cells11193048
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