Cargando…
Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis
Pseudomonas aeruginosa infection is a severe acute suppurative ulcer that engulfs virtually the entire tissue in a short period and leads to devastating destruction. Antibiotic therapy is a common approach for the prophylaxis and treatment of P. aeruginosa infection. However, it is often associated...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563483/ https://www.ncbi.nlm.nih.gov/pubmed/36242070 http://dx.doi.org/10.1186/s12951-022-01653-w |
_version_ | 1784808414548852736 |
---|---|
author | Wang, Hongwei Song, Fangying Feng, Jing Qi, Xia Ma, Li Xie, Lixin Shi, Weiyun Zhou, Qingjun |
author_facet | Wang, Hongwei Song, Fangying Feng, Jing Qi, Xia Ma, Li Xie, Lixin Shi, Weiyun Zhou, Qingjun |
author_sort | Wang, Hongwei |
collection | PubMed |
description | Pseudomonas aeruginosa infection is a severe acute suppurative ulcer that engulfs virtually the entire tissue in a short period and leads to devastating destruction. Antibiotic therapy is a common approach for the prophylaxis and treatment of P. aeruginosa infection. However, it is often associated with serious side effects, complications, and multidrug resistance. Therefore, it has been a long-standing challenge to explore safe and effective methods for controlling P. aeruginosa infection. Herein, tannin-coordinated nanozyme composite-based hybrid hydrogels (TCNH) are developed and characterized for the prophylactic treatment of P. aeruginosa and multidrug-resistant P. aeruginosa infections using mouse keratitis as the animal model. The TCNH eye drops are constructed by photoinitiated free radical polymerization of acetylated gelatin solution containing self-synthesized tannin-coordinated Co(3)O(4)/Ag nanozyme composite. The as-prepared TCNH displays good dispersibility, peroxidase-like activity and in vitro/in vivo biocompatibility. The nanozyme composite in TCNH seems to penetrate the interior of bacteria and exhibited significant broad-spectrum antibacterial activity owing to its intrinsic and nanozymic catalytic properties. Furthermore, TCNH eye drops can be successfully applied to treat P. aeruginosa and multidrug-resistant P. aeruginosa keratitis. The findings of this study reveal the potential of tannin-coordinated nanozyme composite-based hybrid hydrogel eye drops for treating infectious diseases. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01653-w. |
format | Online Article Text |
id | pubmed-9563483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-95634832022-10-15 Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis Wang, Hongwei Song, Fangying Feng, Jing Qi, Xia Ma, Li Xie, Lixin Shi, Weiyun Zhou, Qingjun J Nanobiotechnology Research Pseudomonas aeruginosa infection is a severe acute suppurative ulcer that engulfs virtually the entire tissue in a short period and leads to devastating destruction. Antibiotic therapy is a common approach for the prophylaxis and treatment of P. aeruginosa infection. However, it is often associated with serious side effects, complications, and multidrug resistance. Therefore, it has been a long-standing challenge to explore safe and effective methods for controlling P. aeruginosa infection. Herein, tannin-coordinated nanozyme composite-based hybrid hydrogels (TCNH) are developed and characterized for the prophylactic treatment of P. aeruginosa and multidrug-resistant P. aeruginosa infections using mouse keratitis as the animal model. The TCNH eye drops are constructed by photoinitiated free radical polymerization of acetylated gelatin solution containing self-synthesized tannin-coordinated Co(3)O(4)/Ag nanozyme composite. The as-prepared TCNH displays good dispersibility, peroxidase-like activity and in vitro/in vivo biocompatibility. The nanozyme composite in TCNH seems to penetrate the interior of bacteria and exhibited significant broad-spectrum antibacterial activity owing to its intrinsic and nanozymic catalytic properties. Furthermore, TCNH eye drops can be successfully applied to treat P. aeruginosa and multidrug-resistant P. aeruginosa keratitis. The findings of this study reveal the potential of tannin-coordinated nanozyme composite-based hybrid hydrogel eye drops for treating infectious diseases. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01653-w. BioMed Central 2022-10-14 /pmc/articles/PMC9563483/ /pubmed/36242070 http://dx.doi.org/10.1186/s12951-022-01653-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Hongwei Song, Fangying Feng, Jing Qi, Xia Ma, Li Xie, Lixin Shi, Weiyun Zhou, Qingjun Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title | Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title_full | Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title_fullStr | Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title_full_unstemmed | Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title_short | Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis |
title_sort | tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant pseudomonas aeruginosa keratitis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9563483/ https://www.ncbi.nlm.nih.gov/pubmed/36242070 http://dx.doi.org/10.1186/s12951-022-01653-w |
work_keys_str_mv | AT wanghongwei tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT songfangying tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT fengjing tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT qixia tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT mali tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT xielixin tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT shiweiyun tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis AT zhouqingjun tannincoordinatednanozymecompositebasedhybridhydrogeleyedropsforprophylactictreatmentofmultidrugresistantpseudomonasaeruginosakeratitis |