Cargando…

Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy

Deficiency of estradiol during the menopausal period is an important risk factor for neurodegenerative diseases, including various optic neuropathies. The aim of this study was to evaluate the impact of surgical menopause on the function and survival ratio of RGCs in the rat model of ONC (optic nerv...

Descripción completa

Detalles Bibliográficos
Autores principales: Olakowska, Edyta, Rodak, Piotr, Pacwa, Anna, Machowicz, Joanna, Machna, Bartosz, Lewin-Kowalik, Joanna, Smedowski, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9564175/
https://www.ncbi.nlm.nih.gov/pubmed/36231022
http://dx.doi.org/10.3390/cells11193062
_version_ 1784808576408092672
author Olakowska, Edyta
Rodak, Piotr
Pacwa, Anna
Machowicz, Joanna
Machna, Bartosz
Lewin-Kowalik, Joanna
Smedowski, Adrian
author_facet Olakowska, Edyta
Rodak, Piotr
Pacwa, Anna
Machowicz, Joanna
Machna, Bartosz
Lewin-Kowalik, Joanna
Smedowski, Adrian
author_sort Olakowska, Edyta
collection PubMed
description Deficiency of estradiol during the menopausal period is an important risk factor for neurodegenerative diseases, including various optic neuropathies. The aim of this study was to evaluate the impact of surgical menopause on the function and survival ratio of RGCs in the rat model of ONC (optic nerve crush). We used eight-week-old female Long Evans rats, divided into two main groups depending on the time between ovariectomy procedure (OVA) and euthanasia (two weeks vs. seven weeks), and subgroups—OVA, OVA + ONC, or ONC. Retinal function was assessed with electroretinography (ERG). RGC loss ratio was evaluated using immunolabelling and counting of RGCs. Seven weeks after OVA, the menopause morphologically affected interneurons but not RGC; however, when the ONC procedure was applied, RGCs appeared to be more susceptible to damage in case of deprivation of estrogens. In our analysis, PhNR (photopic negative responses) were severely diminished in the OVA + ONC group. A deprivation of estrogens in menopause results in accelerated retinal neurodegeneration that firstly involves retinal interneurons. The lack of estrogens increases the susceptibility of RGCs to insults.
format Online
Article
Text
id pubmed-9564175
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95641752022-10-15 Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy Olakowska, Edyta Rodak, Piotr Pacwa, Anna Machowicz, Joanna Machna, Bartosz Lewin-Kowalik, Joanna Smedowski, Adrian Cells Article Deficiency of estradiol during the menopausal period is an important risk factor for neurodegenerative diseases, including various optic neuropathies. The aim of this study was to evaluate the impact of surgical menopause on the function and survival ratio of RGCs in the rat model of ONC (optic nerve crush). We used eight-week-old female Long Evans rats, divided into two main groups depending on the time between ovariectomy procedure (OVA) and euthanasia (two weeks vs. seven weeks), and subgroups—OVA, OVA + ONC, or ONC. Retinal function was assessed with electroretinography (ERG). RGC loss ratio was evaluated using immunolabelling and counting of RGCs. Seven weeks after OVA, the menopause morphologically affected interneurons but not RGC; however, when the ONC procedure was applied, RGCs appeared to be more susceptible to damage in case of deprivation of estrogens. In our analysis, PhNR (photopic negative responses) were severely diminished in the OVA + ONC group. A deprivation of estrogens in menopause results in accelerated retinal neurodegeneration that firstly involves retinal interneurons. The lack of estrogens increases the susceptibility of RGCs to insults. MDPI 2022-09-29 /pmc/articles/PMC9564175/ /pubmed/36231022 http://dx.doi.org/10.3390/cells11193062 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olakowska, Edyta
Rodak, Piotr
Pacwa, Anna
Machowicz, Joanna
Machna, Bartosz
Lewin-Kowalik, Joanna
Smedowski, Adrian
Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title_full Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title_fullStr Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title_full_unstemmed Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title_short Surgical Menopause Impairs Retinal Conductivity and Worsens Prognosis in an Acute Model of Rat Optic Neuropathy
title_sort surgical menopause impairs retinal conductivity and worsens prognosis in an acute model of rat optic neuropathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9564175/
https://www.ncbi.nlm.nih.gov/pubmed/36231022
http://dx.doi.org/10.3390/cells11193062
work_keys_str_mv AT olakowskaedyta surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT rodakpiotr surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT pacwaanna surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT machowiczjoanna surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT machnabartosz surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT lewinkowalikjoanna surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy
AT smedowskiadrian surgicalmenopauseimpairsretinalconductivityandworsensprognosisinanacutemodelofratopticneuropathy