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Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa

Heavy metals are unbreakable, and most of them are poisonous to animals and people. Metals are particularly concerning among environmental contaminants since they are less apparent, have extensive effects on ecosystems, are poisonous, and bioaccumulate in ecosystems, biological tissues, and organs....

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Autores principales: Maphuhla, Nontobeko Gloria, Lewu, Francis Bayo, Oyedeji, Opeoluwa Oyehan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565107/
https://www.ncbi.nlm.nih.gov/pubmed/36231352
http://dx.doi.org/10.3390/ijerph191912054
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author Maphuhla, Nontobeko Gloria
Lewu, Francis Bayo
Oyedeji, Opeoluwa Oyehan
author_facet Maphuhla, Nontobeko Gloria
Lewu, Francis Bayo
Oyedeji, Opeoluwa Oyehan
author_sort Maphuhla, Nontobeko Gloria
collection PubMed
description Heavy metals are unbreakable, and most of them are poisonous to animals and people. Metals are particularly concerning among environmental contaminants since they are less apparent, have extensive effects on ecosystems, are poisonous, and bioaccumulate in ecosystems, biological tissues, and organs. Therefore, there is a need to use biological agents and phytoremediation processes such as enzymes because they have a high potential for effectively transforming and detoxifying polluting substances. They can convert pollutants at a detectable rate and are potentially suitable for restoring polluted environments. We investigated heavy metal concentrations in different soil samples collected in four sections in Alice and determined the enzyme activity levels present in the soil. The Pearson correlation analysis was conducted to check whether there was any relationship between heavy metal concentrations and enzyme activities in the soil. Samples were randomly collected in three weeks, and the microwave digestion method was used for sample treatment and preparation. Quantitation was achieved by inductively coupled plasma mass spectrometry (ICP-MS). The enzyme assay through incubation method was implemented for discovering the four selected enzymes (urease, invertase, catalase, and phosphatase), and their activity levels were examined colorimetrically by colorimetry spectrophotometer. The ICP-MS results revealed 16 predominating elements, namely: Al, Ba, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Sr, and Zn, and the presence of a non-mental, which is phosphorus (P), and a metalloid in the form of silicon (Si) in all soil samples. Significant differences in metal concentrations were observed among the collection sites. The Al, Fe, K, Mg, and Ca concentrations were above WHO’s permissible limits. While Ba, Mn, Na, and P were in moderate concentration, Cu, Cr, Co, Zn, Sr, and Ni were in small amounts recorded mostly below the permissible values from WHO. Four soil enzyme activities were determined successfully (urease, invertase, phosphatase, and catalase). A negative non-significant correlation existed between urease, invertase, phosphatase enzyme activity, and the concentration levels of all selected metals (Al, Ba, Ca, Co, Cu, Fe, K, Mg, Mn, Na, Ni, Cr, Sr, and Zn. In contrast, the content of catalase activity was associated non-significantly but positively with the range of selected heavy metals. This study suggests proper monitoring of residences’ areas, which can provide detailed information on the impact of high heavy metal content on people’s health. They are easily dispersed and can accumulate in large quantities in the soil. The necessary implementation of waste management programs will help the municipality adopt a strategy that will promote recycling programs and protect the residence health from this threat.
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spelling pubmed-95651072022-10-15 Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa Maphuhla, Nontobeko Gloria Lewu, Francis Bayo Oyedeji, Opeoluwa Oyehan Int J Environ Res Public Health Article Heavy metals are unbreakable, and most of them are poisonous to animals and people. Metals are particularly concerning among environmental contaminants since they are less apparent, have extensive effects on ecosystems, are poisonous, and bioaccumulate in ecosystems, biological tissues, and organs. Therefore, there is a need to use biological agents and phytoremediation processes such as enzymes because they have a high potential for effectively transforming and detoxifying polluting substances. They can convert pollutants at a detectable rate and are potentially suitable for restoring polluted environments. We investigated heavy metal concentrations in different soil samples collected in four sections in Alice and determined the enzyme activity levels present in the soil. The Pearson correlation analysis was conducted to check whether there was any relationship between heavy metal concentrations and enzyme activities in the soil. Samples were randomly collected in three weeks, and the microwave digestion method was used for sample treatment and preparation. Quantitation was achieved by inductively coupled plasma mass spectrometry (ICP-MS). The enzyme assay through incubation method was implemented for discovering the four selected enzymes (urease, invertase, catalase, and phosphatase), and their activity levels were examined colorimetrically by colorimetry spectrophotometer. The ICP-MS results revealed 16 predominating elements, namely: Al, Ba, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Sr, and Zn, and the presence of a non-mental, which is phosphorus (P), and a metalloid in the form of silicon (Si) in all soil samples. Significant differences in metal concentrations were observed among the collection sites. The Al, Fe, K, Mg, and Ca concentrations were above WHO’s permissible limits. While Ba, Mn, Na, and P were in moderate concentration, Cu, Cr, Co, Zn, Sr, and Ni were in small amounts recorded mostly below the permissible values from WHO. Four soil enzyme activities were determined successfully (urease, invertase, phosphatase, and catalase). A negative non-significant correlation existed between urease, invertase, phosphatase enzyme activity, and the concentration levels of all selected metals (Al, Ba, Ca, Co, Cu, Fe, K, Mg, Mn, Na, Ni, Cr, Sr, and Zn. In contrast, the content of catalase activity was associated non-significantly but positively with the range of selected heavy metals. This study suggests proper monitoring of residences’ areas, which can provide detailed information on the impact of high heavy metal content on people’s health. They are easily dispersed and can accumulate in large quantities in the soil. The necessary implementation of waste management programs will help the municipality adopt a strategy that will promote recycling programs and protect the residence health from this threat. MDPI 2022-09-23 /pmc/articles/PMC9565107/ /pubmed/36231352 http://dx.doi.org/10.3390/ijerph191912054 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maphuhla, Nontobeko Gloria
Lewu, Francis Bayo
Oyedeji, Opeoluwa Oyehan
Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title_full Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title_fullStr Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title_full_unstemmed Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title_short Enzyme Activities in Reduction of Heavy Metal Pollution from Alice Landfill Site in Eastern Cape, South Africa
title_sort enzyme activities in reduction of heavy metal pollution from alice landfill site in eastern cape, south africa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565107/
https://www.ncbi.nlm.nih.gov/pubmed/36231352
http://dx.doi.org/10.3390/ijerph191912054
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