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Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods

There were two analytical methods for the determination of 12 carbonyl compounds (CCs) by using liquid chromatography (LC) coupled with mass spectrometry (MS/MS) and diode array detector (UV/DAD) that were developed and applied to 52 samples that were collected in 10 workplaces. Linearity (0.996 <...

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Autores principales: Castellani, Federica, Antonucci, Arianna, Pindinello, Ivano, Protano, Carmela, Vitali, Matteo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565147/
https://www.ncbi.nlm.nih.gov/pubmed/36231348
http://dx.doi.org/10.3390/ijerph191912052
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author Castellani, Federica
Antonucci, Arianna
Pindinello, Ivano
Protano, Carmela
Vitali, Matteo
author_facet Castellani, Federica
Antonucci, Arianna
Pindinello, Ivano
Protano, Carmela
Vitali, Matteo
author_sort Castellani, Federica
collection PubMed
description There were two analytical methods for the determination of 12 carbonyl compounds (CCs) by using liquid chromatography (LC) coupled with mass spectrometry (MS/MS) and diode array detector (UV/DAD) that were developed and applied to 52 samples that were collected in 10 workplaces. Linearity (0.996 < R(2) < 0.999), intra-day repeatability (0.7 < RSD% < 10), and inter-day repeatability (5 < RSD% < 16) were acceptable for both techniques, but the highest sensibility of the MS/MS method allowed us to correctly quantify 98% of the samples (versus 32% by UV/DAD). The comparison of the concentrations that were obtained by quantifying the same sample with both techniques showed good agreement for acetaldehyde and formaldehyde (0.1 < % deviation < 30) but much higher for the less abundant congeners. In real samples, formaldehyde was the most abundant congener (concentrations between 2.7 and 77 µg m(−3)), followed by acetaldehyde (concentrations between 1.5 and 79 µg m(−3)) and butyraldehyde (concentrations between 0.4 and 13 µg m(−3)). In all the beauty salon samples, instead, the most abundant congener was acetaldehyde (concentrations between 19 and 79 µg m(−3)), probably associated with the use of beauty products. Principal components analysis (PCA) confirms the ubiquitous character of formaldehyde and highlights the influence of minority CCs on different workplaces.
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spelling pubmed-95651472022-10-15 Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods Castellani, Federica Antonucci, Arianna Pindinello, Ivano Protano, Carmela Vitali, Matteo Int J Environ Res Public Health Article There were two analytical methods for the determination of 12 carbonyl compounds (CCs) by using liquid chromatography (LC) coupled with mass spectrometry (MS/MS) and diode array detector (UV/DAD) that were developed and applied to 52 samples that were collected in 10 workplaces. Linearity (0.996 < R(2) < 0.999), intra-day repeatability (0.7 < RSD% < 10), and inter-day repeatability (5 < RSD% < 16) were acceptable for both techniques, but the highest sensibility of the MS/MS method allowed us to correctly quantify 98% of the samples (versus 32% by UV/DAD). The comparison of the concentrations that were obtained by quantifying the same sample with both techniques showed good agreement for acetaldehyde and formaldehyde (0.1 < % deviation < 30) but much higher for the less abundant congeners. In real samples, formaldehyde was the most abundant congener (concentrations between 2.7 and 77 µg m(−3)), followed by acetaldehyde (concentrations between 1.5 and 79 µg m(−3)) and butyraldehyde (concentrations between 0.4 and 13 µg m(−3)). In all the beauty salon samples, instead, the most abundant congener was acetaldehyde (concentrations between 19 and 79 µg m(−3)), probably associated with the use of beauty products. Principal components analysis (PCA) confirms the ubiquitous character of formaldehyde and highlights the influence of minority CCs on different workplaces. MDPI 2022-09-23 /pmc/articles/PMC9565147/ /pubmed/36231348 http://dx.doi.org/10.3390/ijerph191912052 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castellani, Federica
Antonucci, Arianna
Pindinello, Ivano
Protano, Carmela
Vitali, Matteo
Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title_full Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title_fullStr Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title_full_unstemmed Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title_short Determination of Carbonyl Compounds in Different Work Environments: Comparison between LC-UV/DAD and LC–MS/MS Detection Methods
title_sort determination of carbonyl compounds in different work environments: comparison between lc-uv/dad and lc–ms/ms detection methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565147/
https://www.ncbi.nlm.nih.gov/pubmed/36231348
http://dx.doi.org/10.3390/ijerph191912052
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