Screen-Printed Graphite Electrode Modified with Graphene-Co(3)O(4) Nanocomposite: Voltammetric Assay of Morphine in the Presence of Diclofenac in Pharmaceutical and Biological Samples

This work focuses on the development of a novel electrochemical sensor for the determination of morphine in the presence of diclofenac. The facile synthesis of graphene-Co(3)O(4) nanocomposite was performed. The prepared material (graphene-Co(3)O(4) nanocomposite) was analyzed by diverse microscopic and spectroscopic approaches for its crystallinity, composition, and morphology. Concerning the electrochemical determinations, after drop-casting the as-fabricated graphene-Co(3)O(4) nanocomposite on the surface of a screen-printed graphite electrode (SPGE), their electrochemical performance was scrutinized towards the morphine detection. It was also found that an SPGE modified by a graphene-Co(3)O(4) nanocomposite exhibited better electrocatalytic activity for morphine oxidation than unmodified electrode. Under optimal conditions, the differential pulse voltammetry (DPV) was employed to explore the present sensor (graphene-Co(3)O(4)/SPGE), the findings of which revealed a linear dynamic range as broad as 0.02–575.0 µM and a limit of detection (LOD) as narrow as 0.007 μM. The sensitivity was estimated to be 0.4 µM/(µA cm(2)). Furthermore, the graphene-Co(3)O(4)/SPGE sensor demonstrated good analytical efficiency for sensing morphine in the presence of diclofenac in well-spaced anodic peaks. According to the DPV results, this sensor displayed two distinct peaks for the oxidation of morphine and diclofenac with 350 mV potential difference. In addition, the graphene-Co(3)O(4)/SPGE was explored for voltammetric determination of diclofenac and morphine in pharmaceutical and biological specimens of morphine ampoule, diclofenac tablet, and urine, where recovery rates close to 100% were recorded for all of the samples.