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A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution

Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ion...

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Autores principales: Eghbal, Mina, Rozman, Martin, Kononenko, Veno, Hočevar, Matej, Drobne, Damjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565818/
https://www.ncbi.nlm.nih.gov/pubmed/36234621
http://dx.doi.org/10.3390/nano12193493
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author Eghbal, Mina
Rozman, Martin
Kononenko, Veno
Hočevar, Matej
Drobne, Damjana
author_facet Eghbal, Mina
Rozman, Martin
Kononenko, Veno
Hočevar, Matej
Drobne, Damjana
author_sort Eghbal, Mina
collection PubMed
description Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ions in a solution as a reference test chemical. A549 cells were attached to the surface of stainless-steel electrodes. After treatment with ZnCl(2), the morphological changes of the cells and, ultimately, their death and detachment from the electrode surface as cytotoxic effects were detected through changes in the electrical signal. Electrochemical cell-based impedance spectroscopy (ECIS) measurements were conducted with cytotoxicity tests and microscopic observation to investigate the behavior of the A549 cells. As expected, the Zn(2+) ions caused changes in cell confluency and spreading, which were checked by light microscopy, while the cell morphology and attachment pattern were explored by scanning electron microscopy (SEM). The ECIS measurements confirmed the ability of the biosensor to detect the effects of Zn(2+) ions on A549 cells attached to the low-cost stainless-steel surfaces and its potential for use as an inexpensive detector for a broad range of chemicals and nanomaterials in their cytotoxic concentrations.
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spelling pubmed-95658182022-10-15 A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution Eghbal, Mina Rozman, Martin Kononenko, Veno Hočevar, Matej Drobne, Damjana Nanomaterials (Basel) Article Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ions in a solution as a reference test chemical. A549 cells were attached to the surface of stainless-steel electrodes. After treatment with ZnCl(2), the morphological changes of the cells and, ultimately, their death and detachment from the electrode surface as cytotoxic effects were detected through changes in the electrical signal. Electrochemical cell-based impedance spectroscopy (ECIS) measurements were conducted with cytotoxicity tests and microscopic observation to investigate the behavior of the A549 cells. As expected, the Zn(2+) ions caused changes in cell confluency and spreading, which were checked by light microscopy, while the cell morphology and attachment pattern were explored by scanning electron microscopy (SEM). The ECIS measurements confirmed the ability of the biosensor to detect the effects of Zn(2+) ions on A549 cells attached to the low-cost stainless-steel surfaces and its potential for use as an inexpensive detector for a broad range of chemicals and nanomaterials in their cytotoxic concentrations. MDPI 2022-10-06 /pmc/articles/PMC9565818/ /pubmed/36234621 http://dx.doi.org/10.3390/nano12193493 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eghbal, Mina
Rozman, Martin
Kononenko, Veno
Hočevar, Matej
Drobne, Damjana
A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title_full A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title_fullStr A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title_full_unstemmed A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title_short A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
title_sort a549 cell-covered electrodes as a sensing element for detection of effects of zn(2+) ions in a solution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565818/
https://www.ncbi.nlm.nih.gov/pubmed/36234621
http://dx.doi.org/10.3390/nano12193493
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