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A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution
Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ion...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565818/ https://www.ncbi.nlm.nih.gov/pubmed/36234621 http://dx.doi.org/10.3390/nano12193493 |
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author | Eghbal, Mina Rozman, Martin Kononenko, Veno Hočevar, Matej Drobne, Damjana |
author_facet | Eghbal, Mina Rozman, Martin Kononenko, Veno Hočevar, Matej Drobne, Damjana |
author_sort | Eghbal, Mina |
collection | PubMed |
description | Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ions in a solution as a reference test chemical. A549 cells were attached to the surface of stainless-steel electrodes. After treatment with ZnCl(2), the morphological changes of the cells and, ultimately, their death and detachment from the electrode surface as cytotoxic effects were detected through changes in the electrical signal. Electrochemical cell-based impedance spectroscopy (ECIS) measurements were conducted with cytotoxicity tests and microscopic observation to investigate the behavior of the A549 cells. As expected, the Zn(2+) ions caused changes in cell confluency and spreading, which were checked by light microscopy, while the cell morphology and attachment pattern were explored by scanning electron microscopy (SEM). The ECIS measurements confirmed the ability of the biosensor to detect the effects of Zn(2+) ions on A549 cells attached to the low-cost stainless-steel surfaces and its potential for use as an inexpensive detector for a broad range of chemicals and nanomaterials in their cytotoxic concentrations. |
format | Online Article Text |
id | pubmed-9565818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95658182022-10-15 A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution Eghbal, Mina Rozman, Martin Kononenko, Veno Hočevar, Matej Drobne, Damjana Nanomaterials (Basel) Article Electrochemical-based biosensors have the potential to be a fast, label-free, simple approach to detecting the effects of cytotoxic substances in liquid media. In the work presented here, a cell-based electrochemical biosensor was developed and evaluated to detect the cytotoxic effects of Zn(2+) ions in a solution as a reference test chemical. A549 cells were attached to the surface of stainless-steel electrodes. After treatment with ZnCl(2), the morphological changes of the cells and, ultimately, their death and detachment from the electrode surface as cytotoxic effects were detected through changes in the electrical signal. Electrochemical cell-based impedance spectroscopy (ECIS) measurements were conducted with cytotoxicity tests and microscopic observation to investigate the behavior of the A549 cells. As expected, the Zn(2+) ions caused changes in cell confluency and spreading, which were checked by light microscopy, while the cell morphology and attachment pattern were explored by scanning electron microscopy (SEM). The ECIS measurements confirmed the ability of the biosensor to detect the effects of Zn(2+) ions on A549 cells attached to the low-cost stainless-steel surfaces and its potential for use as an inexpensive detector for a broad range of chemicals and nanomaterials in their cytotoxic concentrations. MDPI 2022-10-06 /pmc/articles/PMC9565818/ /pubmed/36234621 http://dx.doi.org/10.3390/nano12193493 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eghbal, Mina Rozman, Martin Kononenko, Veno Hočevar, Matej Drobne, Damjana A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title | A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title_full | A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title_fullStr | A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title_full_unstemmed | A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title_short | A549 Cell-Covered Electrodes as a Sensing Element for Detection of Effects of Zn(2+) Ions in a Solution |
title_sort | a549 cell-covered electrodes as a sensing element for detection of effects of zn(2+) ions in a solution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565818/ https://www.ncbi.nlm.nih.gov/pubmed/36234621 http://dx.doi.org/10.3390/nano12193493 |
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