Cargando…

Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest

The purpose of this study was to determine the impact of localized cooling of the skeletal muscle during rest on mitochondrial related gene expression. Thermal wraps were applied to the vastus lateralis of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while...

Descripción completa

Detalles Bibliográficos
Autores principales: Robins, Larry, Kwon, Monica, McGlynn, Mark L., Rosales, Alejandro M., Pekas, Elizabeth J., Collins, Christopher, Park, Song-Young, Slivka, Dustin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9566196/
https://www.ncbi.nlm.nih.gov/pubmed/36231330
http://dx.doi.org/10.3390/ijerph191912028
_version_ 1784809091669950464
author Robins, Larry
Kwon, Monica
McGlynn, Mark L.
Rosales, Alejandro M.
Pekas, Elizabeth J.
Collins, Christopher
Park, Song-Young
Slivka, Dustin R.
author_facet Robins, Larry
Kwon, Monica
McGlynn, Mark L.
Rosales, Alejandro M.
Pekas, Elizabeth J.
Collins, Christopher
Park, Song-Young
Slivka, Dustin R.
author_sort Robins, Larry
collection PubMed
description The purpose of this study was to determine the impact of localized cooling of the skeletal muscle during rest on mitochondrial related gene expression. Thermal wraps were applied to the vastus lateralis of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while the other did not (RT). Wraps were removed at the 4 h time point and measurements of skin temperature, blood flow, and intramuscular temperature were taken prior to a muscle biopsy. RT-qPCR was used to measure expression of genes associated with mitochondrial development. Skin and muscle temperatures were lower in COLD than RT (p < 0.05). Femoral artery diameter was lower in COLD after 4 h (0.62 ± 0.05 cm, to 0.60 ± 0.05 cm, p = 0.018). Blood flow was not different in COLD compared to RT (259 ± 69 mL·min(–1) vs. 275 ± 54 mL·min(–1), p = 0.20). PGC-1α B and GABPA expression was higher in COLD relative to RT (1.57-fold, p = 0.037 and 1.34-fold, p = 0.006, respectively). There was no difference (p > 0.05) in the expression of PGC-1α, NT-PGC-1α, PGC-1α A, TFAM, ESRRα, NRF1, GABPA, VEGF, PINK1, PARK 2, or BNIP3-L. The impact of this small magnitude of difference in gene expression of PGC-1α B and GABPA without alterations in other genes are unknown. There appears to be only limited impact of local muscle cooling on the transcriptional response related to mitochondrial development.
format Online
Article
Text
id pubmed-9566196
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95661962022-10-15 Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest Robins, Larry Kwon, Monica McGlynn, Mark L. Rosales, Alejandro M. Pekas, Elizabeth J. Collins, Christopher Park, Song-Young Slivka, Dustin R. Int J Environ Res Public Health Article The purpose of this study was to determine the impact of localized cooling of the skeletal muscle during rest on mitochondrial related gene expression. Thermal wraps were applied to the vastus lateralis of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while the other did not (RT). Wraps were removed at the 4 h time point and measurements of skin temperature, blood flow, and intramuscular temperature were taken prior to a muscle biopsy. RT-qPCR was used to measure expression of genes associated with mitochondrial development. Skin and muscle temperatures were lower in COLD than RT (p < 0.05). Femoral artery diameter was lower in COLD after 4 h (0.62 ± 0.05 cm, to 0.60 ± 0.05 cm, p = 0.018). Blood flow was not different in COLD compared to RT (259 ± 69 mL·min(–1) vs. 275 ± 54 mL·min(–1), p = 0.20). PGC-1α B and GABPA expression was higher in COLD relative to RT (1.57-fold, p = 0.037 and 1.34-fold, p = 0.006, respectively). There was no difference (p > 0.05) in the expression of PGC-1α, NT-PGC-1α, PGC-1α A, TFAM, ESRRα, NRF1, GABPA, VEGF, PINK1, PARK 2, or BNIP3-L. The impact of this small magnitude of difference in gene expression of PGC-1α B and GABPA without alterations in other genes are unknown. There appears to be only limited impact of local muscle cooling on the transcriptional response related to mitochondrial development. MDPI 2022-09-23 /pmc/articles/PMC9566196/ /pubmed/36231330 http://dx.doi.org/10.3390/ijerph191912028 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Robins, Larry
Kwon, Monica
McGlynn, Mark L.
Rosales, Alejandro M.
Pekas, Elizabeth J.
Collins, Christopher
Park, Song-Young
Slivka, Dustin R.
Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title_full Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title_fullStr Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title_full_unstemmed Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title_short Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression at Rest
title_sort influence of local muscle cooling on mitochondrial-related gene expression at rest
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9566196/
https://www.ncbi.nlm.nih.gov/pubmed/36231330
http://dx.doi.org/10.3390/ijerph191912028
work_keys_str_mv AT robinslarry influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT kwonmonica influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT mcglynnmarkl influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT rosalesalejandrom influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT pekaselizabethj influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT collinschristopher influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT parksongyoung influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest
AT slivkadustinr influenceoflocalmusclecoolingonmitochondrialrelatedgeneexpressionatrest