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First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan
Coxiellosis, also known as Q fever, is a zoonotic disease caused by Coxiella burnetii, a gram-negative bacterium that exerts a significant deleterious impact on the productive and reproductive capabilities of livestock, severely effecting the economics of this sector. In this study, 448 sera samples...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568511/ https://www.ncbi.nlm.nih.gov/pubmed/36241681 http://dx.doi.org/10.1038/s41598-022-21405-y |
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author | Hussain, Sabir Hussain, Abrar Aziz, Muhammad Umair Song, Baolin Zeb, Jehan Hasib, F. M. Yasir Li, Jun Rehman, Abdul George, David Cabezas-Cruz, Alejandro Sparagano, Olivier |
author_facet | Hussain, Sabir Hussain, Abrar Aziz, Muhammad Umair Song, Baolin Zeb, Jehan Hasib, F. M. Yasir Li, Jun Rehman, Abdul George, David Cabezas-Cruz, Alejandro Sparagano, Olivier |
author_sort | Hussain, Sabir |
collection | PubMed |
description | Coxiellosis, also known as Q fever, is a zoonotic disease caused by Coxiella burnetii, a gram-negative bacterium that exerts a significant deleterious impact on the productive and reproductive capabilities of livestock, severely effecting the economics of this sector. In this study, 448 sera samples from cattle (n = 224) and buffalo (n = 224) were collected from 112 farms in Pakistan and examined for antibodies against C. burnetii using an indirect ELISA. Ticks were also collected from these animals. Serological analysis revealed a 23.66% and 27.23% seroprevalence of Q fever in cattle and buffalo, respectively. Odds ratio (OR) analysis of the factors associated with C. burnetii seropositivity was performed, and a multivariable logistic model identified five main variables associated with the seropositivity for coxiellosis. These were: (i) the absence of acaricide use (OR 5.61; 95% CI 2.97–10.94); (ii) the presence of ticks (OR 3.23; 95% CI 1.87–5.69); (iii) the abortion history during the preceding year on the farm (OR 14.96; 95% CI 8.09–29.34); (iv) the presence of sheep and goats (OR 2.47; 95% CI 1.20–5.35); and (v) the absence of a separate parturition area (OR 3.17; 95% CI 1.76–5.86). This study provides new insights into the seroprevalence of Q fever in large ruminants across seven studied districts of Punjab, Pakistan, also providing baseline data to inform improved herd management and on-farm practices for the prevention and control of Q fever in large ruminants in the region. Results of this work suggest that further molecular investigation of coxiellosis is warranted to provide a more thorough evaluation of C. burnetii epidemiology in Pakistan. |
format | Online Article Text |
id | pubmed-9568511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95685112022-10-16 First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan Hussain, Sabir Hussain, Abrar Aziz, Muhammad Umair Song, Baolin Zeb, Jehan Hasib, F. M. Yasir Li, Jun Rehman, Abdul George, David Cabezas-Cruz, Alejandro Sparagano, Olivier Sci Rep Article Coxiellosis, also known as Q fever, is a zoonotic disease caused by Coxiella burnetii, a gram-negative bacterium that exerts a significant deleterious impact on the productive and reproductive capabilities of livestock, severely effecting the economics of this sector. In this study, 448 sera samples from cattle (n = 224) and buffalo (n = 224) were collected from 112 farms in Pakistan and examined for antibodies against C. burnetii using an indirect ELISA. Ticks were also collected from these animals. Serological analysis revealed a 23.66% and 27.23% seroprevalence of Q fever in cattle and buffalo, respectively. Odds ratio (OR) analysis of the factors associated with C. burnetii seropositivity was performed, and a multivariable logistic model identified five main variables associated with the seropositivity for coxiellosis. These were: (i) the absence of acaricide use (OR 5.61; 95% CI 2.97–10.94); (ii) the presence of ticks (OR 3.23; 95% CI 1.87–5.69); (iii) the abortion history during the preceding year on the farm (OR 14.96; 95% CI 8.09–29.34); (iv) the presence of sheep and goats (OR 2.47; 95% CI 1.20–5.35); and (v) the absence of a separate parturition area (OR 3.17; 95% CI 1.76–5.86). This study provides new insights into the seroprevalence of Q fever in large ruminants across seven studied districts of Punjab, Pakistan, also providing baseline data to inform improved herd management and on-farm practices for the prevention and control of Q fever in large ruminants in the region. Results of this work suggest that further molecular investigation of coxiellosis is warranted to provide a more thorough evaluation of C. burnetii epidemiology in Pakistan. Nature Publishing Group UK 2022-10-14 /pmc/articles/PMC9568511/ /pubmed/36241681 http://dx.doi.org/10.1038/s41598-022-21405-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hussain, Sabir Hussain, Abrar Aziz, Muhammad Umair Song, Baolin Zeb, Jehan Hasib, F. M. Yasir Li, Jun Rehman, Abdul George, David Cabezas-Cruz, Alejandro Sparagano, Olivier First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title | First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title_full | First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title_fullStr | First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title_full_unstemmed | First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title_short | First serological evidence of Q fever in large ruminants and its associated risk factors in Punjab, Pakistan |
title_sort | first serological evidence of q fever in large ruminants and its associated risk factors in punjab, pakistan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568511/ https://www.ncbi.nlm.nih.gov/pubmed/36241681 http://dx.doi.org/10.1038/s41598-022-21405-y |
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