Cargando…

3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study

Synchrotron X-ray computed tomography (SXCT) allows 3D imaging of tissue with a very large field of view and an excellent micron resolution and enables the investigation of muscle fiber atrophy in 3D. The study aimed to explore the 3D micro-architecture of healthy skeletal muscle fibers and muscle f...

Descripción completa

Detalles Bibliográficos
Autores principales: Pingel, Jessica, Kjer, Hans Martin, Biering-Sørensen, Fin, Feidenhans’l, Robert, Dyrby, Tim B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568578/
https://www.ncbi.nlm.nih.gov/pubmed/36241693
http://dx.doi.org/10.1038/s41598-022-21741-z
_version_ 1784809670471319552
author Pingel, Jessica
Kjer, Hans Martin
Biering-Sørensen, Fin
Feidenhans’l, Robert
Dyrby, Tim B.
author_facet Pingel, Jessica
Kjer, Hans Martin
Biering-Sørensen, Fin
Feidenhans’l, Robert
Dyrby, Tim B.
author_sort Pingel, Jessica
collection PubMed
description Synchrotron X-ray computed tomography (SXCT) allows 3D imaging of tissue with a very large field of view and an excellent micron resolution and enables the investigation of muscle fiber atrophy in 3D. The study aimed to explore the 3D micro-architecture of healthy skeletal muscle fibers and muscle fibers at different stages of atrophy (stroke sample = muscle atrophy; spinal cord injury (SCI) sample = severe muscle atrophy). Three muscle samples: a healthy control sample; a stroke sample (atrophic sample), and an SCI sample (severe atrophic sample) were imaged using SXCT, and muscle fiber populations were segmented and quantified for microarchitecture and morphology differences. The volume fraction of muscle fibers was 74.7%, 70.2%, and 35.3% in the healthy, stroke (atrophic), and SCI (severe atrophic) muscle fiber population samples respectively. In the SCI (severe atrophic sample), 3D image analysis revealed fiber splitting and fiber swelling. In the stroke sample (atrophic sample) muscle fiber buckling was observed but was only visible in the 3D analysis. 3D muscle fiber population analysis revealed new insights into the different stages of muscle fiber atrophy not to be observed nor quantified with a 2D histological analysis including fiber buckling, loss of fibers and fiber splitting.
format Online
Article
Text
id pubmed-9568578
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-95685782022-10-16 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study Pingel, Jessica Kjer, Hans Martin Biering-Sørensen, Fin Feidenhans’l, Robert Dyrby, Tim B. Sci Rep Article Synchrotron X-ray computed tomography (SXCT) allows 3D imaging of tissue with a very large field of view and an excellent micron resolution and enables the investigation of muscle fiber atrophy in 3D. The study aimed to explore the 3D micro-architecture of healthy skeletal muscle fibers and muscle fibers at different stages of atrophy (stroke sample = muscle atrophy; spinal cord injury (SCI) sample = severe muscle atrophy). Three muscle samples: a healthy control sample; a stroke sample (atrophic sample), and an SCI sample (severe atrophic sample) were imaged using SXCT, and muscle fiber populations were segmented and quantified for microarchitecture and morphology differences. The volume fraction of muscle fibers was 74.7%, 70.2%, and 35.3% in the healthy, stroke (atrophic), and SCI (severe atrophic) muscle fiber population samples respectively. In the SCI (severe atrophic sample), 3D image analysis revealed fiber splitting and fiber swelling. In the stroke sample (atrophic sample) muscle fiber buckling was observed but was only visible in the 3D analysis. 3D muscle fiber population analysis revealed new insights into the different stages of muscle fiber atrophy not to be observed nor quantified with a 2D histological analysis including fiber buckling, loss of fibers and fiber splitting. Nature Publishing Group UK 2022-10-14 /pmc/articles/PMC9568578/ /pubmed/36241693 http://dx.doi.org/10.1038/s41598-022-21741-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Pingel, Jessica
Kjer, Hans Martin
Biering-Sørensen, Fin
Feidenhans’l, Robert
Dyrby, Tim B.
3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title_full 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title_fullStr 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title_full_unstemmed 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title_short 3D synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
title_sort 3d synchrotron imaging of muscle tissues at different atrophic stages in stroke and spinal cord injury: a proof-of-concept study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568578/
https://www.ncbi.nlm.nih.gov/pubmed/36241693
http://dx.doi.org/10.1038/s41598-022-21741-z
work_keys_str_mv AT pingeljessica 3dsynchrotronimagingofmuscletissuesatdifferentatrophicstagesinstrokeandspinalcordinjuryaproofofconceptstudy
AT kjerhansmartin 3dsynchrotronimagingofmuscletissuesatdifferentatrophicstagesinstrokeandspinalcordinjuryaproofofconceptstudy
AT bieringsørensenfin 3dsynchrotronimagingofmuscletissuesatdifferentatrophicstagesinstrokeandspinalcordinjuryaproofofconceptstudy
AT feidenhanslrobert 3dsynchrotronimagingofmuscletissuesatdifferentatrophicstagesinstrokeandspinalcordinjuryaproofofconceptstudy
AT dyrbytimb 3dsynchrotronimagingofmuscletissuesatdifferentatrophicstagesinstrokeandspinalcordinjuryaproofofconceptstudy