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Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)

Uridine diphosphate glycosyltransferases (UGTs) are enzymes that catalyze glycosylation modifications and play an essential role in regulating plant metabolism. Alfalfa (Medicago sativa L.) is the most important legume in the world due to its high yields and protein content; however, the UGT genes i...

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Autores principales: Yu, Andong, Jiang, Xueqian, Sun, Yan, Hu, Qiannan, Zhu, Xiaoxi, Kang, Junmei, Chen, Lin, Liu, Lin, Hao, Linfeng, Yang, Qingchuan, Long, Ruicai, Li, Mingna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568668/
https://www.ncbi.nlm.nih.gov/pubmed/36254261
http://dx.doi.org/10.3389/fpls.2022.1001206
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author Yu, Andong
Jiang, Xueqian
Sun, Yan
Hu, Qiannan
Zhu, Xiaoxi
Kang, Junmei
Chen, Lin
Liu, Lin
Hao, Linfeng
Yang, Qingchuan
Long, Ruicai
Li, Mingna
author_facet Yu, Andong
Jiang, Xueqian
Sun, Yan
Hu, Qiannan
Zhu, Xiaoxi
Kang, Junmei
Chen, Lin
Liu, Lin
Hao, Linfeng
Yang, Qingchuan
Long, Ruicai
Li, Mingna
author_sort Yu, Andong
collection PubMed
description Uridine diphosphate glycosyltransferases (UGTs) are enzymes that catalyze glycosylation modifications and play an essential role in regulating plant metabolism. Alfalfa (Medicago sativa L.) is the most important legume in the world due to its high yields and protein content; however, the UGT genes in alfalfa have not yet been studied. Identifying UGT genes with metabolic roles in alfalfa is essential for identifying and modifying genetic traits that are relevant to yield and quality. In this study, 90 of the 239 UGT genes identified from the alfalfa “Zhongmu No. 1” genome database were found to be related to secondary metabolism, and a series of gene family characterization analyses were conducted on each. The results demonstrated that all 90 UGT genes were unevenly distributed on eight chromosomes with few introns and that tandem duplications were the crucial driving force expanding the UGT family in alfalfa. Notably, the 90 UGT genes can be clustered into ten evolutionary groups which contain specific PSPG motifs, and genes in these ten groups have specific tissue expressions. This suggests that the UGT genes in each group could have similar glycosylation roles corresponding to analogous secondary metabolites in alfalfa. Additionally, multiple cis-acting elements found in MsUGT promoter regions, such as phytohormone and flavonoids, indicate that 90 UGT members could be induced by these features, which are also related to secondary metabolism. Therefore, our study identified 90 UGT members inten evolutionary groups that are likely related to glycosylation modifications with secondary metabolites in alfalfa. These findings help uncover pivotal regulatory mechanisms associated with secondary metabolism in plant yield and quality and contribute to genetic modification and breeding in alfalfa and other plant species.
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spelling pubmed-95686682022-10-16 Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.) Yu, Andong Jiang, Xueqian Sun, Yan Hu, Qiannan Zhu, Xiaoxi Kang, Junmei Chen, Lin Liu, Lin Hao, Linfeng Yang, Qingchuan Long, Ruicai Li, Mingna Front Plant Sci Plant Science Uridine diphosphate glycosyltransferases (UGTs) are enzymes that catalyze glycosylation modifications and play an essential role in regulating plant metabolism. Alfalfa (Medicago sativa L.) is the most important legume in the world due to its high yields and protein content; however, the UGT genes in alfalfa have not yet been studied. Identifying UGT genes with metabolic roles in alfalfa is essential for identifying and modifying genetic traits that are relevant to yield and quality. In this study, 90 of the 239 UGT genes identified from the alfalfa “Zhongmu No. 1” genome database were found to be related to secondary metabolism, and a series of gene family characterization analyses were conducted on each. The results demonstrated that all 90 UGT genes were unevenly distributed on eight chromosomes with few introns and that tandem duplications were the crucial driving force expanding the UGT family in alfalfa. Notably, the 90 UGT genes can be clustered into ten evolutionary groups which contain specific PSPG motifs, and genes in these ten groups have specific tissue expressions. This suggests that the UGT genes in each group could have similar glycosylation roles corresponding to analogous secondary metabolites in alfalfa. Additionally, multiple cis-acting elements found in MsUGT promoter regions, such as phytohormone and flavonoids, indicate that 90 UGT members could be induced by these features, which are also related to secondary metabolism. Therefore, our study identified 90 UGT members inten evolutionary groups that are likely related to glycosylation modifications with secondary metabolites in alfalfa. These findings help uncover pivotal regulatory mechanisms associated with secondary metabolism in plant yield and quality and contribute to genetic modification and breeding in alfalfa and other plant species. Frontiers Media S.A. 2022-09-30 /pmc/articles/PMC9568668/ /pubmed/36254261 http://dx.doi.org/10.3389/fpls.2022.1001206 Text en Copyright © 2022 Yu, Jiang, Sun, Hu, Zhu, Kang, Chen, Liu, Hao, Yang, Long and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yu, Andong
Jiang, Xueqian
Sun, Yan
Hu, Qiannan
Zhu, Xiaoxi
Kang, Junmei
Chen, Lin
Liu, Lin
Hao, Linfeng
Yang, Qingchuan
Long, Ruicai
Li, Mingna
Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title_full Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title_fullStr Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title_full_unstemmed Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title_short Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)
title_sort genome-wide identification, characterization, and expression analysis of udp-glycosyltransferase genes associated with secondary metabolism in alfalfa (medicago sativa l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568668/
https://www.ncbi.nlm.nih.gov/pubmed/36254261
http://dx.doi.org/10.3389/fpls.2022.1001206
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