An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI

Functional magnetic resonance imaging (fMRI) in mouse brain, paired with spatially and temporally defined manipulations, offers a powerful tool to causally explain the effect of specific neuronal activity on brain network dynamics. Here, we present an optimized protocol to measure cell-type-specific...

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Detalles Bibliográficos
Autores principales: Grimm, Christina, Wenderoth, Nicole, Zerbi, Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568887/
https://www.ncbi.nlm.nih.gov/pubmed/36240060
http://dx.doi.org/10.1016/j.xpro.2022.101761
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author Grimm, Christina
Wenderoth, Nicole
Zerbi, Valerio
author_facet Grimm, Christina
Wenderoth, Nicole
Zerbi, Valerio
author_sort Grimm, Christina
collection PubMed
description Functional magnetic resonance imaging (fMRI) in mouse brain, paired with spatially and temporally defined manipulations, offers a powerful tool to causally explain the effect of specific neuronal activity on brain network dynamics. Here, we present an optimized protocol to measure cell-type-specific contributions to changes in whole-brain dynamics in mice using optogenetics (opto)-fMRI. This protocol details the injection of ChR2-expressing AAV, the implantation of optical fiber, the steps to perform opto-BOLD (blood-oxygenation-level-dependent) fMRI recording, and data analysis. For complete details on the use and execution of this protocol, please refer to Grimm et al. (2021).
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spelling pubmed-95688872022-10-16 An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI Grimm, Christina Wenderoth, Nicole Zerbi, Valerio STAR Protoc Protocol Functional magnetic resonance imaging (fMRI) in mouse brain, paired with spatially and temporally defined manipulations, offers a powerful tool to causally explain the effect of specific neuronal activity on brain network dynamics. Here, we present an optimized protocol to measure cell-type-specific contributions to changes in whole-brain dynamics in mice using optogenetics (opto)-fMRI. This protocol details the injection of ChR2-expressing AAV, the implantation of optical fiber, the steps to perform opto-BOLD (blood-oxygenation-level-dependent) fMRI recording, and data analysis. For complete details on the use and execution of this protocol, please refer to Grimm et al. (2021). Elsevier 2022-10-13 /pmc/articles/PMC9568887/ /pubmed/36240060 http://dx.doi.org/10.1016/j.xpro.2022.101761 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Grimm, Christina
Wenderoth, Nicole
Zerbi, Valerio
An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title_full An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title_fullStr An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title_full_unstemmed An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title_short An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI
title_sort optimized protocol for assessing changes in mouse whole-brain activity using opto-fmri
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568887/
https://www.ncbi.nlm.nih.gov/pubmed/36240060
http://dx.doi.org/10.1016/j.xpro.2022.101761
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