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Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides

Effect of dipyridamole (DIP) at concentrations up to 1 mM on fluorescent characteristics of light-harvesting complexes LH2 and LH1, as well as on conditions of photosynthetic electron transport chain in the bacterial chromatophores of Rba. sphaeroides was investigated. DIP was found to affect effici...

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Autores principales: Knox, Peter P., Lukashev, Eugene P., Korvatovskii, Boris N., Seifullina, Nuranija Kh., Goryachev, Sergey N., Allakhverdiev, Elvin S., Paschenko, Vladimir Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568914/
https://www.ncbi.nlm.nih.gov/pubmed/36273882
http://dx.doi.org/10.1134/S0006297922100078
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author Knox, Peter P.
Lukashev, Eugene P.
Korvatovskii, Boris N.
Seifullina, Nuranija Kh.
Goryachev, Sergey N.
Allakhverdiev, Elvin S.
Paschenko, Vladimir Z.
author_facet Knox, Peter P.
Lukashev, Eugene P.
Korvatovskii, Boris N.
Seifullina, Nuranija Kh.
Goryachev, Sergey N.
Allakhverdiev, Elvin S.
Paschenko, Vladimir Z.
author_sort Knox, Peter P.
collection PubMed
description Effect of dipyridamole (DIP) at concentrations up to 1 mM on fluorescent characteristics of light-harvesting complexes LH2 and LH1, as well as on conditions of photosynthetic electron transport chain in the bacterial chromatophores of Rba. sphaeroides was investigated. DIP was found to affect efficiency of energy transfer from the light-harvesting complex LH2 to the LH1–reaction center core complex and to produce the long-wavelength (“red”) shift of the absorption band of light-harvesting bacteriochlorophyll molecules in the IR spectral region at 840-900 nm. This shift is associated with the membrane transition to the energized state. It was shown that DIP is able to reduce the photooxidized bacteriochlorophyll of the reaction center, which accelerated electron flow along the electron transport chain, thereby stimulating generation of the transmembrane potential on the chromatophore membrane. The results are important for clarifying possible mechanisms of DIP influence on the activity of membrane-bound functional proteins. In particular, they might be significant for interpreting numerous therapeutic effects of DIP.
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spelling pubmed-95689142022-10-16 Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides Knox, Peter P. Lukashev, Eugene P. Korvatovskii, Boris N. Seifullina, Nuranija Kh. Goryachev, Sergey N. Allakhverdiev, Elvin S. Paschenko, Vladimir Z. Biochemistry (Mosc) Article Effect of dipyridamole (DIP) at concentrations up to 1 mM on fluorescent characteristics of light-harvesting complexes LH2 and LH1, as well as on conditions of photosynthetic electron transport chain in the bacterial chromatophores of Rba. sphaeroides was investigated. DIP was found to affect efficiency of energy transfer from the light-harvesting complex LH2 to the LH1–reaction center core complex and to produce the long-wavelength (“red”) shift of the absorption band of light-harvesting bacteriochlorophyll molecules in the IR spectral region at 840-900 nm. This shift is associated with the membrane transition to the energized state. It was shown that DIP is able to reduce the photooxidized bacteriochlorophyll of the reaction center, which accelerated electron flow along the electron transport chain, thereby stimulating generation of the transmembrane potential on the chromatophore membrane. The results are important for clarifying possible mechanisms of DIP influence on the activity of membrane-bound functional proteins. In particular, they might be significant for interpreting numerous therapeutic effects of DIP. Pleiades Publishing 2022-10-14 2022 /pmc/articles/PMC9568914/ /pubmed/36273882 http://dx.doi.org/10.1134/S0006297922100078 Text en © Pleiades Publishing, Ltd. 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Knox, Peter P.
Lukashev, Eugene P.
Korvatovskii, Boris N.
Seifullina, Nuranija Kh.
Goryachev, Sergey N.
Allakhverdiev, Elvin S.
Paschenko, Vladimir Z.
Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title_full Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title_fullStr Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title_full_unstemmed Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title_short Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides
title_sort effect of dipyridamole on membrane energization and energy transfer in chromatophores of rba. sphaeroides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9568914/
https://www.ncbi.nlm.nih.gov/pubmed/36273882
http://dx.doi.org/10.1134/S0006297922100078
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