Cargando…
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
BACKGROUND: CRISPR-Cas based diagnostic assays provide a portable solution which bridges the benefits of qRT-PCR and serological assays in terms of portability, specificity and ease of use. CRISPR-Cas assays are rapidly fieldable, specific and have been rigorously validated against a number of targe...
Autores principales: | Mann, James G., Pitts, R. Jason |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569017/ https://www.ncbi.nlm.nih.gov/pubmed/36241974 http://dx.doi.org/10.1186/s12859-022-04968-5 |
Ejemplares similares
-
CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA
por: Jedrzejczyk, D. J., et al.
Publicado: (2022) -
CRISPR-Cas-Docker: web-based in silico docking and machine learning-based classification of crRNAs with Cas proteins
por: Park, Ho-min, et al.
Publicado: (2023) -
Structural constraints and enzymatic promiscuity in the Cas6-dependent generation of crRNAs
por: Reimann, Viktoria, et al.
Publicado: (2017) -
Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency
por: Li, Bin, et al.
Publicado: (2017) -
Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system
por: Li, Yingnan, et al.
Publicado: (2023)