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A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking
ApoB-100 is a member of a large lipid transfer protein superfamily and is one of the main apolipoproteins found on low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) particles. Despite its clinical significance for the development of cardiovascular disease, there is limited inform...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569473/ https://www.ncbi.nlm.nih.gov/pubmed/36232786 http://dx.doi.org/10.3390/ijms231911480 |
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author | Jeiran, Kianoush Gordon, Scott M. Sviridov, Denis O. Aponte, Angel M. Haymond, Amanda Piszczek, Grzegorz Lucero, Diego Neufeld, Edward B. Vaisman, Iosif I. Liotta, Lance Baranova, Ancha Remaley, Alan T. |
author_facet | Jeiran, Kianoush Gordon, Scott M. Sviridov, Denis O. Aponte, Angel M. Haymond, Amanda Piszczek, Grzegorz Lucero, Diego Neufeld, Edward B. Vaisman, Iosif I. Liotta, Lance Baranova, Ancha Remaley, Alan T. |
author_sort | Jeiran, Kianoush |
collection | PubMed |
description | ApoB-100 is a member of a large lipid transfer protein superfamily and is one of the main apolipoproteins found on low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) particles. Despite its clinical significance for the development of cardiovascular disease, there is limited information on apoB-100 structure. We have developed a novel method based on the “divide and conquer” algorithm, using PSIPRED software, by dividing apoB-100 into five subunits and 11 domains. Models of each domain were prepared using I-TASSER, DEMO, RoseTTAFold, Phyre2, and MODELLER. Subsequently, we used disuccinimidyl sulfoxide (DSSO), a new mass spectrometry cleavable cross-linker, and the known position of disulfide bonds to experimentally validate each model. We obtained 65 unique DSSO cross-links, of which 87.5% were within a 26 Å threshold in the final model. We also evaluated the positions of cysteine residues involved in the eight known disulfide bonds in apoB-100, and each pair was measured within the expected 5.6 Å constraint. Finally, multiple domains were combined by applying constraints based on detected long-range DSSO cross-links to generate five subunits, which were subsequently merged to achieve an uninterrupted architecture for apoB-100 around a lipoprotein particle. Moreover, the dynamics of apoB-100 during particle size transitions was examined by comparing VLDL and LDL computational models and using experimental cross-linking data. In addition, the proposed model of receptor ligand binding of apoB-100 provides new insights into some of its functions. |
format | Online Article Text |
id | pubmed-9569473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95694732022-10-17 A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking Jeiran, Kianoush Gordon, Scott M. Sviridov, Denis O. Aponte, Angel M. Haymond, Amanda Piszczek, Grzegorz Lucero, Diego Neufeld, Edward B. Vaisman, Iosif I. Liotta, Lance Baranova, Ancha Remaley, Alan T. Int J Mol Sci Article ApoB-100 is a member of a large lipid transfer protein superfamily and is one of the main apolipoproteins found on low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) particles. Despite its clinical significance for the development of cardiovascular disease, there is limited information on apoB-100 structure. We have developed a novel method based on the “divide and conquer” algorithm, using PSIPRED software, by dividing apoB-100 into five subunits and 11 domains. Models of each domain were prepared using I-TASSER, DEMO, RoseTTAFold, Phyre2, and MODELLER. Subsequently, we used disuccinimidyl sulfoxide (DSSO), a new mass spectrometry cleavable cross-linker, and the known position of disulfide bonds to experimentally validate each model. We obtained 65 unique DSSO cross-links, of which 87.5% were within a 26 Å threshold in the final model. We also evaluated the positions of cysteine residues involved in the eight known disulfide bonds in apoB-100, and each pair was measured within the expected 5.6 Å constraint. Finally, multiple domains were combined by applying constraints based on detected long-range DSSO cross-links to generate five subunits, which were subsequently merged to achieve an uninterrupted architecture for apoB-100 around a lipoprotein particle. Moreover, the dynamics of apoB-100 during particle size transitions was examined by comparing VLDL and LDL computational models and using experimental cross-linking data. In addition, the proposed model of receptor ligand binding of apoB-100 provides new insights into some of its functions. MDPI 2022-09-29 /pmc/articles/PMC9569473/ /pubmed/36232786 http://dx.doi.org/10.3390/ijms231911480 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jeiran, Kianoush Gordon, Scott M. Sviridov, Denis O. Aponte, Angel M. Haymond, Amanda Piszczek, Grzegorz Lucero, Diego Neufeld, Edward B. Vaisman, Iosif I. Liotta, Lance Baranova, Ancha Remaley, Alan T. A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title | A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title_full | A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title_fullStr | A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title_full_unstemmed | A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title_short | A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking |
title_sort | new structural model of apolipoprotein b100 based on computational modeling and cross linking |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569473/ https://www.ncbi.nlm.nih.gov/pubmed/36232786 http://dx.doi.org/10.3390/ijms231911480 |
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