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Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study

Actinic keratosis is an intraepithelial proliferation of atypical keratinocytes that could progress into invasive squamous cell carcinoma. Most evidence suggests an important role of the dermal matrix metalloproteinases in the progression of atypical skin epithelial lesions. We evaluated the clinica...

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Autores principales: Campione, Elena, Di Prete, Monia, Di Raimondo, Cosimo, Costanza, Gaetana, Palumbo, Vincenzo, Garofalo, Virginia, Mazzilli, Sara, Franceschini, Chiara, Dika, Emi, Bianchi, Luca, Orlandi, Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569516/
https://www.ncbi.nlm.nih.gov/pubmed/36232651
http://dx.doi.org/10.3390/ijms231911351
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author Campione, Elena
Di Prete, Monia
Di Raimondo, Cosimo
Costanza, Gaetana
Palumbo, Vincenzo
Garofalo, Virginia
Mazzilli, Sara
Franceschini, Chiara
Dika, Emi
Bianchi, Luca
Orlandi, Augusto
author_facet Campione, Elena
Di Prete, Monia
Di Raimondo, Cosimo
Costanza, Gaetana
Palumbo, Vincenzo
Garofalo, Virginia
Mazzilli, Sara
Franceschini, Chiara
Dika, Emi
Bianchi, Luca
Orlandi, Augusto
author_sort Campione, Elena
collection PubMed
description Actinic keratosis is an intraepithelial proliferation of atypical keratinocytes that could progress into invasive squamous cell carcinoma. Most evidence suggests an important role of the dermal matrix metalloproteinases in the progression of atypical skin epithelial lesions. We evaluated the clinical efficacy of three different therapeutic modalities (a medical device containing 0.8% piroxicam cream and 50+ sunscreen, photodynamic therapy, and ingenol mebutate gel) to treat suspicious actinic keratoses, which were biopsied for histopathological examination and then analyzed for the expression of matrix metalloproteinases by immunohistochemistry. Clinical, dermoscopic, and reflectance confocal microscopy evaluations revealed a gradual decrease in all standard scores validated for actinic keratosis assessment at the end of the treatments. From a histopathological point of view, we documented the substantial restoration of normal skin architecture, while the immunohistochemical evaluation of matrix metalloproteinases showed a reduction in expression in the treated skin lesions compared to the baseline. As actinic keratoses are considered the precursors of squamous cell carcinoma, their treatment is crucial to prevent the development of a more aggressive disease. Our study monitored the evolution of actinic keratoses subjected to three different topical therapies, with the value of correlating clinical and histopathological findings. Moreover, as the matrix metalloproteinases are largely recognized factors involved in the pathogenesis and evolution of actinic keratosis to squamous cell carcinoma, the demonstration by immunohistochemistry of a reduction in their expression after the treatments adds new valuable concern to the field.
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spelling pubmed-95695162022-10-17 Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study Campione, Elena Di Prete, Monia Di Raimondo, Cosimo Costanza, Gaetana Palumbo, Vincenzo Garofalo, Virginia Mazzilli, Sara Franceschini, Chiara Dika, Emi Bianchi, Luca Orlandi, Augusto Int J Mol Sci Article Actinic keratosis is an intraepithelial proliferation of atypical keratinocytes that could progress into invasive squamous cell carcinoma. Most evidence suggests an important role of the dermal matrix metalloproteinases in the progression of atypical skin epithelial lesions. We evaluated the clinical efficacy of three different therapeutic modalities (a medical device containing 0.8% piroxicam cream and 50+ sunscreen, photodynamic therapy, and ingenol mebutate gel) to treat suspicious actinic keratoses, which were biopsied for histopathological examination and then analyzed for the expression of matrix metalloproteinases by immunohistochemistry. Clinical, dermoscopic, and reflectance confocal microscopy evaluations revealed a gradual decrease in all standard scores validated for actinic keratosis assessment at the end of the treatments. From a histopathological point of view, we documented the substantial restoration of normal skin architecture, while the immunohistochemical evaluation of matrix metalloproteinases showed a reduction in expression in the treated skin lesions compared to the baseline. As actinic keratoses are considered the precursors of squamous cell carcinoma, their treatment is crucial to prevent the development of a more aggressive disease. Our study monitored the evolution of actinic keratoses subjected to three different topical therapies, with the value of correlating clinical and histopathological findings. Moreover, as the matrix metalloproteinases are largely recognized factors involved in the pathogenesis and evolution of actinic keratosis to squamous cell carcinoma, the demonstration by immunohistochemistry of a reduction in their expression after the treatments adds new valuable concern to the field. MDPI 2022-09-26 /pmc/articles/PMC9569516/ /pubmed/36232651 http://dx.doi.org/10.3390/ijms231911351 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Campione, Elena
Di Prete, Monia
Di Raimondo, Cosimo
Costanza, Gaetana
Palumbo, Vincenzo
Garofalo, Virginia
Mazzilli, Sara
Franceschini, Chiara
Dika, Emi
Bianchi, Luca
Orlandi, Augusto
Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title_full Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title_fullStr Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title_full_unstemmed Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title_short Topical Treatment of Actinic Keratosis and Metalloproteinase Expression: A Clinico-Pathological Retrospective Study
title_sort topical treatment of actinic keratosis and metalloproteinase expression: a clinico-pathological retrospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569516/
https://www.ncbi.nlm.nih.gov/pubmed/36232651
http://dx.doi.org/10.3390/ijms231911351
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