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Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis
Environmental factors that have been linked to an increased endometriosis risk include exposure to di-(2-ethylhexyl)-phthalate (DEHP), an endocrine disruptor. This study aims to investigate whether DEHP in vitro exposure in primary endometrial stromal cells (EnSC), primary endometrial epithelial cel...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569573/ https://www.ncbi.nlm.nih.gov/pubmed/36232341 http://dx.doi.org/10.3390/ijms231911041 |
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author | Gonzalez-Martin, Roberto Palomar, Andrea Medina-Laver, Yassmin Quiñonero, Alicia Domínguez, Francisco |
author_facet | Gonzalez-Martin, Roberto Palomar, Andrea Medina-Laver, Yassmin Quiñonero, Alicia Domínguez, Francisco |
author_sort | Gonzalez-Martin, Roberto |
collection | PubMed |
description | Environmental factors that have been linked to an increased endometriosis risk include exposure to di-(2-ethylhexyl)-phthalate (DEHP), an endocrine disruptor. This study aims to investigate whether DEHP in vitro exposure in primary endometrial stromal cells (EnSC), primary endometrial epithelial cells (EnEC), and the human endometrial adenocarcinoma cell line Ishikawa properly mimics alterations described in the eutopic endometrium of women with endometriosis. Primary EnSC and EnEC, isolated from six fertile egg donors, and Ishikawa cells were exposed to DEHP (0.1, 1, and 10 µM) and were assessed for viability, endometriosis markers (IL-6, VEGF-A, HOXA10, EZH2, and LSD1), steroid receptor gene expressions (ER-1, ER-2, PR-T, PR-B, and PGRMC1), and invasive capacity. Viability after 72 h of DEHP exposure was not significantly affected. None of the endometriosis markers studied were altered after acute DEHP exposure, nor was the expression of steroid receptors. The invasive capacity of EnSC was significantly increased after 10 µM of DEHP exposure. In conclusion, acute DEHP exposure in primary endometrial cells does not fully phenocopy the changes in the viability, expression of markers, or steroidal receptors described in endometriosis. However, the significant increase in EnSC invasiveness observed after DEHP exposure could be a link between DEHP exposure and increased endometriosis likelihood. |
format | Online Article Text |
id | pubmed-9569573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95695732022-10-17 Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis Gonzalez-Martin, Roberto Palomar, Andrea Medina-Laver, Yassmin Quiñonero, Alicia Domínguez, Francisco Int J Mol Sci Article Environmental factors that have been linked to an increased endometriosis risk include exposure to di-(2-ethylhexyl)-phthalate (DEHP), an endocrine disruptor. This study aims to investigate whether DEHP in vitro exposure in primary endometrial stromal cells (EnSC), primary endometrial epithelial cells (EnEC), and the human endometrial adenocarcinoma cell line Ishikawa properly mimics alterations described in the eutopic endometrium of women with endometriosis. Primary EnSC and EnEC, isolated from six fertile egg donors, and Ishikawa cells were exposed to DEHP (0.1, 1, and 10 µM) and were assessed for viability, endometriosis markers (IL-6, VEGF-A, HOXA10, EZH2, and LSD1), steroid receptor gene expressions (ER-1, ER-2, PR-T, PR-B, and PGRMC1), and invasive capacity. Viability after 72 h of DEHP exposure was not significantly affected. None of the endometriosis markers studied were altered after acute DEHP exposure, nor was the expression of steroid receptors. The invasive capacity of EnSC was significantly increased after 10 µM of DEHP exposure. In conclusion, acute DEHP exposure in primary endometrial cells does not fully phenocopy the changes in the viability, expression of markers, or steroidal receptors described in endometriosis. However, the significant increase in EnSC invasiveness observed after DEHP exposure could be a link between DEHP exposure and increased endometriosis likelihood. MDPI 2022-09-20 /pmc/articles/PMC9569573/ /pubmed/36232341 http://dx.doi.org/10.3390/ijms231911041 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gonzalez-Martin, Roberto Palomar, Andrea Medina-Laver, Yassmin Quiñonero, Alicia Domínguez, Francisco Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title | Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title_full | Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title_fullStr | Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title_full_unstemmed | Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title_short | Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis |
title_sort | endometrial cells acutely exposed to phthalates in vitro do not phenocopy endometriosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569573/ https://www.ncbi.nlm.nih.gov/pubmed/36232341 http://dx.doi.org/10.3390/ijms231911041 |
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