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Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry

The role of extracellular vesicles (EVs) as mediators of cell-to-cell communication in cancer progression is widely recognized. In vitro studies are routinely performed on 2D culture models, but recent studies suggest that 3D cultures could represent a more valid model. Human ovarian cancer cells CA...

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Autores principales: Giusti, Ilaria, Poppa, Giuseppina, D’Ascenzo, Sandra, Esposito, Letizia, Vitale, Anna Rita, Calvisi, Giuseppe, Dolo, Vincenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569704/
https://www.ncbi.nlm.nih.gov/pubmed/36233083
http://dx.doi.org/10.3390/ijms231911782
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author Giusti, Ilaria
Poppa, Giuseppina
D’Ascenzo, Sandra
Esposito, Letizia
Vitale, Anna Rita
Calvisi, Giuseppe
Dolo, Vincenza
author_facet Giusti, Ilaria
Poppa, Giuseppina
D’Ascenzo, Sandra
Esposito, Letizia
Vitale, Anna Rita
Calvisi, Giuseppe
Dolo, Vincenza
author_sort Giusti, Ilaria
collection PubMed
description The role of extracellular vesicles (EVs) as mediators of cell-to-cell communication in cancer progression is widely recognized. In vitro studies are routinely performed on 2D culture models, but recent studies suggest that 3D cultures could represent a more valid model. Human ovarian cancer cells CABA I were cultured by the hanging drop method to form tumor spheroids, that were moved to low adhesion supports to observe their morphology by Scanning Electron Microscopy (SEM) and to isolate the EVs. EVs release was verified by SEM and their identity confirmed by morphology (Transmission Electron Microscopy, TEM), size distribution (Nanoparticles Tracking Analysis), and markers (CD63, CD9, TSG-101, Calnexin). CABA I form spheroids with a clinically relevant size, above 400 μm; they release EVs on their external surface and also trap “inner” EVs. They also produce vasculogenic mimicry-like tubules, that bulge from the spheroid and are composed of a hollow lumen delimited by tumor cells. CABA I can be grown as multicellular spheroids to easily isolate EVs. The presence of features typical of in vivo tumors (inner entrapped EVs and vasculogenic mimicry) suggests their use as faithful experimental models to screen therapeutic drugs targeting these pro-tumorigenic processes.
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spelling pubmed-95697042022-10-17 Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry Giusti, Ilaria Poppa, Giuseppina D’Ascenzo, Sandra Esposito, Letizia Vitale, Anna Rita Calvisi, Giuseppe Dolo, Vincenza Int J Mol Sci Article The role of extracellular vesicles (EVs) as mediators of cell-to-cell communication in cancer progression is widely recognized. In vitro studies are routinely performed on 2D culture models, but recent studies suggest that 3D cultures could represent a more valid model. Human ovarian cancer cells CABA I were cultured by the hanging drop method to form tumor spheroids, that were moved to low adhesion supports to observe their morphology by Scanning Electron Microscopy (SEM) and to isolate the EVs. EVs release was verified by SEM and their identity confirmed by morphology (Transmission Electron Microscopy, TEM), size distribution (Nanoparticles Tracking Analysis), and markers (CD63, CD9, TSG-101, Calnexin). CABA I form spheroids with a clinically relevant size, above 400 μm; they release EVs on their external surface and also trap “inner” EVs. They also produce vasculogenic mimicry-like tubules, that bulge from the spheroid and are composed of a hollow lumen delimited by tumor cells. CABA I can be grown as multicellular spheroids to easily isolate EVs. The presence of features typical of in vivo tumors (inner entrapped EVs and vasculogenic mimicry) suggests their use as faithful experimental models to screen therapeutic drugs targeting these pro-tumorigenic processes. MDPI 2022-10-04 /pmc/articles/PMC9569704/ /pubmed/36233083 http://dx.doi.org/10.3390/ijms231911782 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giusti, Ilaria
Poppa, Giuseppina
D’Ascenzo, Sandra
Esposito, Letizia
Vitale, Anna Rita
Calvisi, Giuseppe
Dolo, Vincenza
Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title_full Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title_fullStr Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title_full_unstemmed Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title_short Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
title_sort cancer three-dimensional spheroids mimic in vivo tumor features, displaying “inner” extracellular vesicles and vasculogenic mimicry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569704/
https://www.ncbi.nlm.nih.gov/pubmed/36233083
http://dx.doi.org/10.3390/ijms231911782
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